α-硫辛酸对体外培养的黑素细胞影响的实验研究

目的　建立正常人表皮黑素细胞体外培养体系 ,研究α 硫辛酸对体外培养的黑素细胞的影响。方法　采用MTT法测定α 硫辛酸对黑素细胞活力的影响 ,以左旋多巴为底物测定酪氨酸酶活性 ,NaOH裂解法测定黑素合成 ,并与茶多酚的结果进行比较。结果　α 硫辛酸对黑素细胞活力有抑制作用 ,能使细胞增殖力降低 ,酪氨酸酶活性减弱 ,黑素合成减少。结论　α 硫辛酸对体外培养的黑素细胞增殖、酪氨酸酶活性及黑素生成都有抑制作用 ,这为临床应用α 硫辛酸治疗色素性疾病提供了实验依据。

Effects of α-Lopoic Acid on Cultured Human Melanocytes in Vitro

Objective Establishing the culture of human normal melanocytes in vitro,and studying the effects of α-lopoic acid on cultured human melanocytes.Methods MTT method,NaOH-assay were employed to measure the cell viability,melanin synthesis of melanocytes;tyrosinase activity was measured by utilization of L-Dopa as the substrate.Furthermore,the effects of aloesin on melanocytes were compared with that of tea polyphenols.Results α-Lopoic acid induced a mild effect on melanocytic viability,while it showed a significant decrease in the cell counts, melanin synthesis and tyrosinase activity.Conclusion α-Lopoic acid can inhibit melanocytic proliferation, melanin synthesis and tyrosinase activity.It is worth to study later.It make valid to make use of α-lopoic acid for treatment of the pigmented skin diseases.