酒精性肝损伤大鼠肠道屏障功能改变及谷氨酰胺的保护作用观察

目的 观察酒精性肝损伤大鼠肠道屏障功能的改变，并探讨谷氨酰胺的保护作用。方法 36只SD大鼠随机分为正常对照组（A组）、模型组（B组）和谷氨酰胺治疗组（C组），分别检测肠道细菌易位率、肠粘膜通透性，同时观察血生化、肝脏和末段回肠粘膜病理学改变。结果 与A组比，B组大鼠血清AST和ALT水平显著升高（AST：256．6±50．8U／L对175．2±16．2U／L，P〈0．05；ALT：86．5±5．4U／L对53．7±13．2U／L，P〈0．05），C组无显著变化（P〉0．05）；B组肠道细菌易位率明显增高（62．5％，P〈0．05），C组无显著变化（35．0％，P〉0．05）；B组肠粘膜通透性显著升高（235．1±26．4ml·min^-1·cm^2对30．1±33．6ml·min^-1·cm^2,P〈0．05），C组肠粘膜通透性比B组明显下降（190．9±19．8ml·min^-1·cm^2对235．1±26．4ml·min^-1·cm^2,P〈0．05）：B组大鼠末段回肠病理损伤明显加重（3．8±0．9对0．4±0．5，P〈0．05），C组比B组明显下降（2．7±0．7对3．8±0．9，P〈0．05）。结论 大鼠酒精性肝损伤伴有肠道屏障功能改变，谷氨酰胺对大鼠酒精性肝损伤及肠道屏障功能具有双重保护作用。

Protection of glutamine on intestinal barrier function in ethanol-induced liver injury in rats

Objective To investigate the changes of intestinal barrier function and protection of glutamine on chronic ethanol-induced liver injury in rats. Methods 36 SD rats were divided randomly into group A （normal control）, group B （model） and group C （glutamine-treated） and the changes in liver biochemistry, intestinal mucosal permeability,bacterial translocation and intestinal mucosa pathohistology were detected,respectively. Results The serum AST（256.6±50.8 U/L vs. 175.2±16.2 U/L,P〈0.05） and ALT levels（86.5±5.4 U/L vs. 53.7±13.2U/L,P〈0.05）,the intestinal mucosal permeability （62.5% vs. 0.0%,P〈0.05）,and the bacterial translocation rate of intestinal tract （235.1±26.4 ml·min^-1·cm^2 vs. 30.1±33.6 ml·min^-1·cm^2,P〈0.05） increased obviously,and the pathological injury of intestinal mucosa （3.8±0.9 vs 0.4±0.5, P〈0.05） aggravated in group B as compared to in group A;while all the parameters in group C were alleviated as compared to in group B. Conclusion In experimental ethanol-induced liver injury in rats,glutamine has a protective role in liver and intestinal barrier injuries.