C-erbB-2,p53,N-ras EXPRESSION IN HEPATOCELLULAR CARCINOMA

To assess the relationship between C-erbB-2, p53,N-ras status at a premalignant stage and in HCC, theauthors studied the imunohistological expression of thesegenes in HCC, liver cirrhosis and in the adjacent normalresected Iiver tissue, using monoclonal antibody tomutated p53 and activated C-erbB-2, N-ras. C-erbB-2was expressed in 97.1 % (35/36) of HCC and 100% (18/18)of helpatic cirrhosis, low level C-erbB-2 expression wasobserved in 2/14 (14.3% of normal liver specimens;The positive incidence of overexpression of mutant p53protein in HCC and hepatic cirrhosis were 55.6%(20/36)and 66.7%(10/18) respectively; 29 (76.5%) specimens ofHCC and 16 (88.9%) of hepatic cirrhosis were positive forN-ras protein. The overexpression of the three oncogeneproteins were significantly higher than that of normalliver tissues (P<0.01). These results indicated thatactivated C-erbB-2, N-ras and altered p53 genes may havea role in human HCC Pathogenesis through promiting thedevelopment of HCC from hepatic cirrhosis and thepro

C-erbB-2, p53, N-ras EXPRESSION IN HEPATO-CELLULAR CARCINOMA

To assess the relationship between C-erbB-2, p53, N-ras status at a premalignant stage and in HCC, the authors studied the imunohistological expression of these genes in HCC, liver cirrhosis and in the adjacent normal resected liver tissue, using monoclonal antibody to mutated p53 and activated C-erbB-2, N-ras. C-erbB-2 was expressed in 97.1% (35/36) of HCC and 100% (18/18) of hepatic cirrhosis, low level C-erbB-2 expression was observed in 2/14 (14.3%) of normal liver specimens; The positive incidence of overexpression of mutant p53 protein in HCC and hepatic cirrhosis were 55.6% (20/36) and 66.7% (10/18) respectively; 29 (76.5%) specimens of HCC and 16 (88.9%) of hepatic cirrhosis were positive for N-ras protein. The overexpression of the three oncogene proteins were significantly higher than that of normal liver tissues (P<0.01). These results indicated that activated C-erbB-2, N-ras and altered p53 genes may have a role in human HCC pathogenesis through promiting the development of HCC from hepatic cirrhosis and the progression of HCC.