端侧神经吻合后再生轴突计数和截面积测定

目的：研究端侧神经吻合后再生轴突的成熟情况。方法：以耳大神经制成端侧吻合的神经模型。将16只新西兰兔按手术的时间分为1、4、7、14、28、42、56、84d8个观察组，每组2只动物。利用HE染色及电子计算机图像分析实验结果。结果：再生轴突的横截面积逐渐增加。再生轴突的通过率随吻合手术时间的延长不断增加，28d时，少量再生的轴突长入受神经，其再生轴突横截面积恢复率是27.7%、42d为38.4%、56d时达61.2%和84d的63.3%。结论：端侧神经吻合后再生的纤维逐步成熟，该修复方法具有一定的临床运用价值。

The Numbers and the Cross Section Area of Regenerating Nerve Axon after End-to-side Neurorrhaphy

Objective: To study the mature situation of regenerating axon after end to side neurorrhaphy.Methods:A rabbit model of the greater auricular nerve end to side anastomosis was established.16 New Zealand rabbits were randomly divided into1 ,4 ,7 ,14 ,28 ,42 ,56 and 84 day groups.The nerve sample of regenerating axon was stained with HE,and the results were examined by image analysis technique with computer.Results:The cross section area of the regenerating axon increased gradually along with elapse of postoperation.The area of regeneration nerve axon was 27.7%,38.4%,61.2% and 63.3% on 28 ,42 ,56 and 84 day respectively.Conclusion:The regenerative nerve fiber maturated step by step after end to side neurorrhaphy,the technique of nerve anastomosis is effective for repairing nerve injuries.