Diesel exhaust particles induce endothelial dysfunction in apoE-/- mice |
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Authors: | Hansen Christian S Sheykhzade Majid Møller Peter Folkmann Janne Kjaergaard Amtorp Ole Jonassen Thomas Loft Steffen |
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Institution: | Department of Environmental and Occupational Health, Institute of Public Health, University of Copenhagen, ?ster Farimagsgade 5, Building 5B, 2nd Floor, 1014 Copenhagen K, Denmark. |
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Abstract: | BACKGROUND: Particulate air pollution can aggravate cardiovascular disease by mechanisms suggested to involve translocation of particles to the bloodstream and impairment of endothelial function, possibly dependent on present atherosclerosis. AIM: We investigated the effects of exposure to diesel exhaust particles (DEP) in vivo and ex vivo on vasomotor functions in aorta from apoE(-/-) mice with slight atherosclerosis and from normal apoE(+/+) mice. METHODS: DEP 0, 0.5 or 5 mg/kg bodyweight in saline was administered i.p. The mice were sacrificed 1 h later and aorta ring segments were mounted on wire myographs. Segments from unexposed mice were also incubated ex vivo with 0, 10 and 100 microg DEP/ml before measurement of vasomotor functions. RESULTS: Exposure to 0.5 mg/kg DEP in vivo caused a decrease in the endothelium-dependent acetylcholine elicited vasorelaxation in apoE(-/-) mice, whereas the response was enhanced in apoE(+/+) mice. No significant change was observed after administration of 5 mg/kg DEP. In vivo DEP exposure did not affect constriction induced by K(+) or phenylephrine. In vitro exposure to 100 microg DEP/ml enhanced acetylcholine-induced relaxation and attenuated phenylephrine-induced constriction. Vasodilation induced by sodium nitroprusside was not affected by any DEP exposure. CONCLUSION: Exposure to DEP has acute effect on vascular functions. Endothelial dysfunction possibly due to decreased NO production as suggested by decreased acetylcholine-induced vasorelaxation and unchanged sodium nitroprusside response can be induced by DEP in vivo only in vessels of mice with some atherosclerosis. |
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Keywords: | ACh acetylcholine DEP diesel exhaust particles PE phenylephrine PM particulate matter PM2 5 particulate matter with diameter less than 2 5 μm PGF2α prostaglandin F2α PSS physiological saline solution SNP sodium nitroprusside |
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