A novel real-time polymerase chain reaction for identification of Salmonella enterica subspecies enterica |
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| Authors: | Hopkins Katie L Lawson Andy J Connell Steve Peters Tansy M de Pinna Elizabeth |
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| Affiliation: | Laboratory of Gastrointestinal Pathogens, Health Protection Agency Centre for Infections, 61 Colindale Avenue, NW9 5EQ, United Kingdom. katie.hopkins@hpa.org.uk |
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| Abstract: | Salmonella enterica subspecies enterica (subspecies I) causes the majority of infections in humans and homeothermic animals. We present a real-time polymerase chain reaction assay targeting the hilA gene that demonstrates 97.9% specificity and 99.9% sensitivity for rapid and reliable identification of subspecies I, offering savings in time and labor over traditional methods. |
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| Keywords: | Salmonella enterica subspecies enterica 5′ Nuclease real-time PCR hilA |
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