HPLC测定千斤肾安宁片中的人参皂苷Rg1、Rb1及三七皂苷R1含量

目的:建立高效液相法同时测定千斤肾安宁片(千斤拔、红参、三七,等)中人参皂苷Rg1,Rb1及三七皂苷R1的含量测定方法。方法:用D101型大孔吸附树脂层析加正丁醇萃取纯化制剂中的人参皂苷Rg1、Rb1及三七皂苷R1等有效成分,并以HPLC法进行含量检测。结果:人参皂苷Rg1、Rb1和三七皂苷R1线性范围分别为1.82~10.92μg,1.74~10.44μg,0.36~2.16μg。该方法回收率Rg1为97.28%(RSD=0.72%),Rb1为101.15%(RSD=0.46%),R1为99.11%(RSD=1.30%)。结论:本测定方法简便可行、重复性好,可用于本制剂中各有效成分的含量测定。

Determination of ginseng saponin Rg1, Rb1 and notoginseng saponin R1 in Qian-jin Shenanning Pills by HPLC

AIM:To establish a method of determining effective components in Qianjin Shenanning Pills(Flemingia Philippinensis Merr.et Rolfe,Radix et Rhizoma Ginseng rubra,Radix et Rhizowa Notoginseng,etc). METHODS: The contents of ginseng saponin Rg_1,Rb_1 and notoginseng saponin R_1 were determined by HPLC. RESULTS: The four effective components could be separated and purified by macroporous adsorptive resins of D_(101) and n-butanol,and their contents could be determined. CONCLUSION: The method is simple,feasible and reproducibility is good,it can be used as quality control in medicinal preparation.