抗表皮生长因子受体单克隆抗体联合5-氟尿嘧啶、健择对胰腺癌的作用

目的 观察抗表皮生长因子受体(EGFR)单克隆抗体联合5-氟尿嘧啶(5-Fu)、健择对胰腺癌的作用.方法 将人胰腺癌肿瘤细胞悬液注射于裸鼠背部皮下,建立肿瘤模型.干预组腹腔注射鼠抗人EGFR单克隆抗体(MMAb-2)、5-Fu、健择及其配伍联合用药;对照组注射生理盐水.干预4周后处死裸鼠,评价干预效果.结果 MMAb-2单独应用对胰腺癌细胞增殖有抑制作用(P<0.05),MMAb-2联合应用5-Fu、健择对胰腺癌细胞增殖的抑制作用明显增强(P<0.05),MMAb-2组裸鼠体质量及其白细胞计数明显高于化疗组(P<0.05).结论 抗EGFR抗体单独应用有确切的抗肿瘤疗效;与化疗联合应用可以增效;对裸鼠机体免疫功能无明显毒性.

The effect of anti-EGFR monoclonal antibody ,5-Fu and gemdtabine therapy on the growth of human pancreatic cancer cells in nude mice model

Objective To study the effect of anti-EGFR monoclonal antibody (MMAb-2) therapy on the growth of pancreatic cancer cells in combination with chemotherapeutic agent. Methods A tumor cell suspension (SW1990) was injected into the subcutaneous dorsa of mice in the proximal tnidline. The treatment groups received intraperitoneal injections of MMAb-2 or 5-Fu or gemcitabine or MMAb-2,5-Fu and gemcitabine in combination respectively. The control group only received intraperitoneal injection of saline. After 4 weeks for treatment, the mice were killed. Results The inhibition ratio of proliferation of pancreatic tumor cells was increased evidently by 5-Fu,gemcitabine in combination with MMAb-2 (P<0.05). Conclusion The MMAb-2 could enhance significantly the sensitivity of the cyto-inhibition of the chemotherapeutic agents on pancreatic tumor cells,and improve the chemotherapeutic effects of pancreatic cancer cells.