高压氧对人脐带华通胶间充质干细胞移植心肌梗死大鼠炎性因子的作用

目的探讨高压氧对移植干细胞在梗死心肌组织局部的炎性状况。方法选择SD大鼠65只,随机分为假手术组5只,心肌梗死组15只,干细胞移植组15只,高压氧组15只,高压氧+干细胞移植组(联合组)15只。建立心肌梗死大鼠模型,模型建立后第1天进行高压氧治疗30d。心肌梗死后第7天进行人脐带华通胶间充质干细胞移植,分别于心肌梗死前、心肌梗死后1、7、14、28d采集血清,ELISA法检测血清TNF-a、白细胞介素1β(IL-1β)、IL-6水平,采用EdU染色法,标记人脐带华通胶间充质干细胞,免疫荧光法检测移植细胞存活及增殖率。结果除外联合组14d时IL-6,联合组3、14、28d时TNF-a、IL-6和IL-1β较心肌梗死组、高压氧组、干细胞移植组明显降低(P<0.05);与干细胞移植组比较,联合组干细胞移植后7、21d心肌组织局部干细胞数量及增殖率明显增高(80.6%vs 55.0%,79.1%vs 34.3%,P<0.05)。结论高压氧能够显著降低梗死心肌组织中的炎性因子水平,有效改善移植干细胞生存的微环境,提高移植干细胞在受损心肌局部的生存及增殖率。

Effect of hyperbaric oxygen on inflammatory factors in myocardial infarction rats following MSC transplantation

Objective To study the effect of hyperbaric oxygen on local inflammation in infracted heart tissue of rats following MSC transplantation.Methods Seventy SD rats were randomly divided into sham operation group(n=5),myocardial infarction(MI)group(n=15),MSC transplantation group(n=15),hyperbaric oxygen group(n=15),hyperbaric oxygen plus MSC transplantation group(combination group,n=15).One day after a rat MI model was established,the rats received hyperbaric oxygen treatment for 30 days.Human umbilical cord Wharton jelly MSC were transplanted into the rats on day 7after MI.Serum levels of TNF-α,IL-1βand IL-6were measured by ELISA before MI and on days 1,7,14 and 28after MI.Human Wharton's jelly MSC were labelled with EdU.Proliferation and survival rates of MSC were assayed using immunofluorescence method.Results The serum levels of TNF-α,IL-6and IL-1βwere significantly lower in combination group than in MI group,hyperbaric oxygen group,MSC transplantation group on days 3,14 and 28after MI(P<0.05).The number and proliferation rate of MSC were significantly higher in combination group than in MSC transplantation group on days 7and 21 after MSC transplantation(80.6%vs 55.0%,79.1%vs 34.3%,P<0.05).Conclusion Hyperbaric oxygen can significantly improve the microenrionment,survival and proliferation rate of transplanted MSC in infarcted myocardial tissue by reducing the inflammation factors level.