体外培养获取高纯度O-2A祖细胞

目的获取较高纯度的O-2A祖细胞,并观察在不同的实验条件下O-2A祖细胞的分化作用。方法 根据星形胶质细胞、O-2A祖细胞的生长时间差异,细胞的粘附特性不同,采用振荡和差速贴壁法结合Ⅰ型星形胶质细胞条件培养液,培养、分离纯化O-2A祖细胞。结果培养的o-2A祖细胞胞体呈椭圆形,有典型的双极突起,少数呈三极突起;经免疫组织化学染色鉴定细胞的纯度达90％。在有血清的情况下,O-2A祖细胞定向分化为Ⅱ型星形胶质细胞;在无血清的情况下,其定向分化为少突胶质细胞。结论通过振荡法和Ⅰ型星形胶质细胞条件培养液结合差速贴壁法分离纯化培养O-2A祖细胞是一种可行的培养方法。

Gaining high purified oligodendrocyte/type-2 astrocyte progenitors in culture

Objective To gain high purified oligodendrocyte/type-2 astrocyte progenitors(O-2A progenitors)in culture, and to observe the differentiation of oligodendrocyte/type-2 astrocyte progenitors on different conditions. Methods Based on the different properties of cellular adhesions and developmental time-course, O-2A progenitors were isolated and purified with a standard shaking method and the differential adhesion method combining with the type-1 astrocyte conditioned medium. Results The purified cells presented typical shape which their bodies were oval and had two processes. Identified by immunohistochemistry, the purity of O-2A progenitors was 90% , and in serum-containing cultures these OA-2 progenitors differentiated into type-2 astrocytes, whereas in serum-free they differentiated into oligodendrocytes. Conclusion A method that acquired enough oligodendrocyte/type-2 astrocyte progenitors was established.