Role of NMDA and non-NMDA receptors in synaptic transmission in rat piriform cortex

Summary The pharmacology of synaptic transmission was studied in slices of rat piriform cortex using the selective non-NMDA glutamate receptor antagonist 6,7-dinitroquinoxaline-2,3-dione (DNQX) and the selective NMDA receptor antagonist D-2-amino-5-phosphonopentanoate (D-AP5). DNQX produced a dose-dependent blockade of synaptic transmission at both lateral olfactory tract and associational system synapses with half-maximal effects at about 2.5 M. D-AP5 had no significant effects on field potentials recorded in medium containing 2.5 mM Mg⁺⁺. However in low Mg⁺⁺ (100–200 M) medium, D-AP5 did reduce a slow component of postsynaptic responses in both synaptic systems. In Mg⁺⁺-free medium, 20 M DNQX did not completely block transmission; the remaining response components were blocked by D-AP5. These results suggest that normal synaptic transmission in the two main inputs to the superficial layers of piriform cortex is mediated by non-NMDA receptors but that NMDA receptors can also participate under conditions where the Mg⁺⁺ block of the NMDA channel is alleviated.