环氧合酶-2对人膀胱移行细胞癌血管内皮生长因子及血管形成的影响

目的 探讨环氧合酶-2(cox-2)对人膀胱移行细胞癌(BTCC)血管内皮生长因子(VEGF)及血管生成的影响.方法 应用免疫组化染色技术检测61例BTCC和7例正常膀胱组织中cox-2、VEGF的表达和微血管密度(MVD).结果 COX-2主要集中在肿瘤细胞胞质内,阳性表达率为55.74%(34/61),VEGF阳性表达率为59.02%(36/61),两者在正常膀胱组织中没有表达,COX-2、VEGF的表达强度与肿瘤的病理分级和分期有关.cox-2与VEGF的表达显著相关(r_s＝ 0.563,P＜0.01).cox-2与VEGF均阳性的膀胱癌组织MVD为76.45±20.60,明显高于两者均阴性者的38.29±15.05,差异有统计学意义(P＜0.01).结论 COX-2与VEGF在人BTCC组织中表达增高,表达强度与肿瘤的分级和分期有关.COX-2可能通过诱导VEGF表达而促进BTCC血管形成.

The effects of cydooxygenase-2 expression on vascular endothelial growth factor and angiogenesis in human bladder transitional cell carcinoma

Objective To investigate the effects of cyclooxygenase 2 COX-2 expression on vascular endothelial growth factor (VEGF) and angiogenesis in human bladder transitional cell carcinoma (BTCC). Method The protein expression of COX-2,VEGF and microvascular density (MVD) was examined by immunohistochemical staining in 61 cases with BTCC and 7 normal bladder mucosa. Results COX-2 protein was mainly localized at cytoplasm of malignant cell,positive rate was 55.74%(34/61),VEGF expression was detected in 59.02% (36/61),but was not expressed in normal bladder mucosa. The intensity of COX-2 and VEGF expression was associated with tumor grade and stage. The expression of COX-2 was significantly correlated with VEGF (r_s＝ 0.563,P＜ 0.01). MVD of bladder carcinoma with positive expression of COX-2 and VEGF was significantly higher than those without expression of COX-2 and VEGF (76.45 ± 20.60 vs 38.29 ± 15.05,P ＜ 0.01). Conclusions The expressions of COX-2 and VEGF were enhanced in human BTCC,and associated with the malignant degree of the tumor,indicating that COX-2 may play a key role in the development and progression of BTCC. COX-2 may stimulate angiogenesis through VEGF upregulation.