氧氟沙星全身用药与局部用药在兔眼通透性比较研究

目的:分别测定全身和局部应用氧氟沙星后不同时间房水和玻璃体中药物浓度,比较这两种用药方法眼部穿透性 的差异。方法:48只日本大耳白兔随机均分为12组,其中5组每只白兔静脉注射氧氟沙星葡萄糖注射液10mg／kg,另5组每只白 兔滴泰利必妥眼液5分钟1次,共5次,剩余2组为空白对照。两种用药方法均在用药后15,30,45,60,120分钟取房水和 玻璃体。采用反向高效液相色谱法进行测定。结果:各时间点的房水氧氟沙星浓度明显高于玻璃体,其中局部用药后玻璃体氧氟 沙星含量无,全身用药玻璃体中可测得氧氟沙星。结论:全身用药局部用药方式的氧氟沙星的前房穿透性好,全身用药氧氟沙星 可穿透血视网膜屏障,这表明预防和治疗感染性眼内炎全身应用氧氟沙星可为首选。

Aqueous Humor and Vitreous Humor Penetration of Rabbits following Intravenous and Topical Ofloxacin

Objective: To compare the eye penetration of ofloxacin on intravenous and topical way after to determine the concentration and dynamic changes of ofloxacin in aqueous humor and vitreous humor. Methods: Forty-eight Japanese big ear white rabbits were randomly divided into twelve groups. The aqueous humor and vitreous humor were collected at 15,30,45,60andl20minutes following intravenous injection of 10mg/kg of ofloxacin in 5 groups and topical tarivid drop every 15 minutes for 5 times in another 5 groups.The rest groups are blank ones.All concentration of ofloxacin was determined by the RP-HPLC. Results: Aqueous humor concentration of ofloxacin was significantly highter than vitreous humor of that at the same period. Nothing was found in the vitreous humor of topical ofloxacin groups. On the other side, ofloxacin was found in the vitreous humor of intravenous ofloxacin groups. Conclusion: Ofloxacin has the good eye penetration by intravenous way that can pass through the blood-retinal barrier, which suggests intravenous ofloxacin could be the first choice in the prevention and treatment of endophthalmitis.