| 乙型肝炎病毒前C区变异对HepG2细胞HLA—I表达的影响 |
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| 引用本文： | 陈伟红 林裕龙 等. 乙型肝炎病毒前C区变异对HepG2细胞HLA—I表达的影响[J]. 中华肝脏病杂志, 2002, 10(5): 351-353 |
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| 作者姓名： | 陈伟红 林裕龙 等 |
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| 作者单位： | 陈伟红（510515,广州,第一军医大学南方医院传染科） 林裕龙（510515,广州,第一军医大学南方医院传染科） 骆抗先（510515,广州,第一军医大学南方医院传染科） 侯金林（510515,广州,第一军医大学南方医院传染科） |
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| 摘 要： | 目的 研究乙型肝炎病毒（HBV）前C区热点变异对宿主细胞HLA－I分子表达的影响。方法 构建HBV前C区野毒株及A83、A83／A86变异株真核表达质粒，转染HepG2细胞，鉴定目的基因在宿主细胞中的生物活性，流式细胞术检测HLA－I分子的表达。结果 PCR和ELISA法分析能检测到目的DNA片段和HBeAg，转染细胞表达HLA－I分子的平均荧光强度不同，野毒株为1.3，前C区变异后平均荧光强度增加，尤以A83变异表达增强显著（17．6），A83／A86为7．3。结论 前C热点变异后宿主细胞HLA－I分子的表达为上调。
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| 关 键 词： | 乙型肝炎病毒 前C区变异 基因变异 HLA－I分子 乙型肝炎 |
| 修稿时间： | 2001-11-28 |
Influence of mutation in HBV precore region on the expression of HLA-I in HepG2 cells] |
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| Weihong Chen,Yulong Lin,Kangxian Luo,Jinlin Hou. Influence of mutation in HBV precore region on the expression of HLA-I in HepG2 cells][J]. Chinese journal of hepatology, 2002, 10(5): 351-353 |
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| Authors: | Weihong Chen Yulong Lin Kangxian Luo Jinlin Hou |
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| Affiliation: | Department of Infectious Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. |
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| Abstract: | OBJECTIVE: To study the influence of host cellular HLA-I molecules expression by HBV precore region mutants. METHODS: The plasmids of the wild type of HBV precore region and the mutants of A83 and A83/A86 were constructed, and then transected into HepG2 cells. The biological activity of HBV precore gene in the host cells was identified. The expression of HLA-I molecules was detected by flow cytometric analysis. RESULTS: DNA segments similar with HBV precore gene size and HBeAg were detected by PCR and ELISA, respectively. The fluorescence intensity of HLA-I on host cells was different: wild type being 1.3; A83, 17.6; and A83/A86, 7.3. CONCLUSIONS: HBV precore gene hot-spot mutants in vitro can increase the expression of HLA-I molecules on host cells. |
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