| 不同频次腹腔注射促红细胞生成素对脑缺血急性期大鼠海马神经元保护作用的比较 |
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| 引用本文： | 王晓斌,宁显忠,王晓强,李志超.不同频次腹腔注射促红细胞生成素对脑缺血急性期大鼠海马神经元保护作用的比较[J].中风与神经疾病杂志,2010,27(7). |
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| 作者姓名： | 王晓斌 宁显忠 王晓强 李志超 |
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| 作者单位： | 1. 辽宁医学院,辽宁,锦州,121001
2. 辽宁医学院附属第三医院神经内科,辽宁,锦州,121001 |
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| 摘 要： | 目的探讨最佳的促红细胞生成素(EPO)给药频次,更有效地保护脑缺血急性期大鼠海马神经元。方法将80只雄性SD大鼠随机分为3组:正常组、模型组、治疗组。除正常组外,其余2组均制成脑缺血再灌注模型;治疗组又分为3个亚组,各亚组之间EPO给药方法相同(腹腔注射)、剂量也相同(3000IU/kg),仅给药频次不同,3次给药组为缺血即刻给1次,缺血再灌后4h给1次,以及缺血再灌后12h给1次;2次给药组为缺血即刻给1次、缺血再灌4h给1次;1次给药组仅为缺血即刻给1次。以上5组大鼠均在缺血再灌24h后制作标本,分别用于HE染色观察病理形态变化、免疫组化染色分析海马Bcl-2、Bax表达;另外用Western Blot测定海马组织Caspase-3的表达。结果 3个亚组对比模型组,Bax表达量显著下降(P<0.05);2次给药组对比模型组,Bcl-2的表达量明显升高(P<0.05);2次给药组与3次给药组对比模型组Caspase-3的表达量显著下降(P<0.05)。结论 EPO能够通过抑制凋亡来保护海马神经元,并且2次给药组具有明显的优势,由此可知,2次给药组接近最佳的给药方法。
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| 关 键 词： | 促红细胞生成素 脑缺血 Bcl-2 Bax Caspase-3 |
Comparision on the protection of hippocampal neuron of rats in different frequency intraperitoneal injection of EPO within acute phase of cerebral ischemia |
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| WANG Xiao-bin,NING Xian-zhong,WANG Xiao-qiang,et al..Comparision on the protection of hippocampal neuron of rats in different frequency intraperitoneal injection of EPO within acute phase of cerebral ischemia[J].Journal of Apoplexy and Nervous Diseases,2010,27(7). |
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| Authors: | WANG Xiao-bin NING Xian-zhong WANG Xiao-qiang |
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| Institution: | WANG Xiao-bin,NING Xian-zhong,WANG Xiao-qiang,et al. (Liaoning Medical College,Jinzhou 121001,China) |
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| Abstract: | Objective To explore the best frequency of EPO administration to get more effective protection of hippocampal neuron of rats in early period of acute cerebral ischemia. Methods 80 male SD rats were randomly divided into 3 groups:normal group,ischemia-reperfusion group and treatment group. In addition to the normal group,the other two groups were made of cerebral ischemia-reperfusion model,meantime the treatment group was divided into three sub-groups. Among the various sub-groups,EPO administration (intrape... |
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| Keywords: | EPO Cerebral ischemia Bcl-2 Bax Caspase-3 |
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