电针对大鼠脑缺血-再灌注过程中体感诱发电位P1-N1、N1-P2峰峰值变化的影响

目的研究大鼠脑缺血及再灌注过程中体感诱发电位的动态变化,以及电针干预对这一动态变化的影响。方法 50只雄性SD大鼠随机分为正常组、假缺血组、假缺血＋电针组、模型组、模型＋电针组;线栓法制作大鼠右侧大脑中动脉脑缺血-再灌注模型,缺血90 min,再灌注225 min;电针大鼠风池穴20 min,停止10 min,再电针20 min;各组分别于处理前、术后30 min、术后60 min、再灌注期间及实验结束时检测大鼠体感诱发电位;分析P1-N1、N1-P2峰峰值。结果正常组、假缺血组和假缺血＋电针组大鼠P1-N1、N1-P2峰峰值变化在监测时段内没有显著差异。模型组、模型＋电针组缺血30 min P1-N1、N1-P2峰峰值出现明显下降;缺血60 min时该峰峰值出现明显恢复;缺血90 min拔出栓线血液再灌注后,模型组P1-N1、N1-P2峰峰值再次出现明显下降,并延续到实验结束;模型＋电针组再灌注的同时给予电针干预,P1-N1、N1-P2峰峰值逐渐恢复至正常水平。结论大鼠脑缺血-再灌注过程中P1-N1、N1-P2峰峰值出现＂降低-恢复-再降低＂的动态变化,电针风池穴可特异性地逆转再灌注P1-N1、N1-P2峰峰值降低。

Effect of Electroacupuncture on Somatosensory Evoked Potential P1-N1 and N1-P2 Peak-to-peak Amplitudes During Cerebral Ischemia and Reperfusion in Rats

Objective To investigate dynamic changes in somatosensory evoked potentials during cerebral ischemia and reperfusion in rats and the effect of electroacupuncture intervention on the changes.Methods Fifty male SD rats were randomly divided into normal,sham ischemia,sham ischemia plus electroacupuncture,model and model plus electroacupuncture groups.A rat model of cerebral ischemia and reperfusion was made by 90 min thread occlusion of the right middle cerebral artery and 225 min reperfusion.Electroacupuncture was performed at rat point Fengchi（GB 20） for 20 min,stopped for 10 min and again for 20 min.Rat somatosensory evoked potentials were examined before treatment,at 30 min and 60 min after operation,during reperfusion and at the end of experiment.P1-N1 and N1-P2 peak-to-peak amplitudes were analyzed.Results There were no significant differences in P1-N1 and N1-P2 peak-to-peak amplitudes during the monitoring process between the normal,sham ischemia and sham ischemia plus electroacupuncture groups.P1-N1 and N1-P2 peak-to-peak amplitudes decreased markedly at 30 min after the artery occlusion and recovered to a large degree at 60 min after the artery occlusion in the model and model plus electroacupuncture groups.During reperfusion following the removal of the intravascular thread at 90 min after the artery occlusion,P1-N1 and N1-P2 peak-to-peak amplitudes decreased markedly again in the model group,which continued to the end of the experiment.During the reperfusion,P1-N1 and N1-P2 peak-to-peak amplitudes return gradually to a normal level in the model plus electroacupuncture group given electroacupuncture at the same time.Conclusions During cerebral ischemia and reperfusion in rats,P1-N1 and N1-P2 peak-to-peak amplitudes have dynamic changes of ＂decrease-recovery-decrease again＂.Electroacupuncture at point Fengchi can specifically reverse decreases in P1-N1 and N1-P2 peak-to-peak amplitudes during the reperfusion.