康复训练对脑梗死大鼠学习记忆与健侧海马突触体胞浆游离Ca2+浓度的影响

目的　探讨康复训练影响脑梗死大鼠学习记忆能力与健侧海马突触体胞浆游离钙离子浓度SCF〔Ca2 〕i的关系。方法　 2 4只Wistar大鼠分为正常组、康复组和模型组 ,每组 8只 ,应用Fura 2荧光探针测定健侧海马神经元SCF〔Ca2 〕i的变化。结果　康复组、模型组和正常组在静息状态下SCF〔Ca2 〕i比较 ,差异无显著性意义 (P >0 .0 5 ) ;在KCl去极化状态下 ,康复组SCF〔Ca2 〕i明显高于模型组和正常组 (P <0 .0 5 )。相应的Y迷宫分辨学习的习得和反映记忆能力的一次性被动回避反应测试能力均优于模型组 (P <0 .0 5 )。结论　康复训练改善脑梗死大鼠学习记忆与海马神经元SCF〔Ca2 〕i的变化密切相关。

Effects of rehabilitation training on learning and memory and intrasynaptosomal (Ca2+)i in hippocampus of rats with cerebral infarction

Objective To explore the effects of rehabilitation training on the learning and memory, as well as intrasynaptosomal free (Ca 2 )i in neurons of the hippocampus in rats with cerebral infarction. Methods Twenty-four Wistar rats were randomly and equally divided into 3 groups: a normal group, a rehabilitation group and a model group. The intrasynaptosomal free (Ca 2 )i was assayed by use of Fura-2 as a fluorescent probe. Results No significant difference was found among the 3 groups(P>0.05), with regard to the concentration of the free (Ca 2 )i in hippocampus in quiescent state. However, in the state of KCl depolarization, the intrasynaptosomal free (Ca 2 )i in the hippocampus of rehabilitation group was found significantly higher than that of the model group and the normal group (P<0.05). The Y labyrinth test revealed better ability of learning and memory in the rehabilitation group comparing with the model group, as indicated by the one-off passive elusion reaction test for the learning and memory ability. Conclusion The improvement of learning ability and memory of the rats with cerebral infarction caused by rehabilitation training might be closely associated with the changes of the intrasynaptosomal free (Ca 2 )i in hippocampus neurons.