| 二硫化碳对邻苯三酚自氧化化学发光体系及超氧阴离子的影响 |
| |
| 引用本文： | Jian L,Chen Q. 二硫化碳对邻苯三酚自氧化化学发光体系及超氧阴离子的影响[J]. 中华预防医学杂志, 2000, 34(3): 162-164 |
| |
| 作者姓名： | Jian L Chen Q |
| |
| 作者单位： | 浙江大学公共卫生学院职业中毒研究室 |
| |
| 摘 要： | 目的 探讨二硫化碳（ＣＳ２）对邻苯三酚－鲁米诺（ＰＡ－Ｌ）自氧化化学发光体系以及对超氧阴离子产生的影响。方法 应用ＰＡ－Ｌ化学发光体系观察ＣＳ２的发光动力学曲线及超氧化物歧化酶（ＳＯＤ）对发光强度的抑制作用。结果 不同浓度ＣＳ２（１０、４０、８０ｍｇ／ｍｌ）与乙醇溶剂对照组比较,均能使ＰＡ－Ｌ体系化学发光强度增强,峰时延迟,呈明显的剂量－效应关系,其中ＣＳ２浓度为８０ｍｇ／ｍｌ时化学发光峰值高于无
|
| 关 键 词： | 二硫化碳 化学发光 邻苯三酚 超氧阴离子 |
Effect of carbon disulfide on pyrogallol- luminol auto-oxidation chemiluminescence system and superoxide anion |
| |
| Jian L,Chen Q. Effect of carbon disulfide on pyrogallol- luminol auto-oxidation chemiluminescence system and superoxide anion[J]. Chinese Journal of Preventive Medicine, 2000, 34(3): 162-164 |
| |
| Authors: | Jian L Chen Q |
| |
| Affiliation: | Research Laboratory of Occupational Poisoning, School of Public Health, Zhejiang University, Hangzhou 310031, China. |
| |
| Abstract: | OBJECTIVE: To study the effect of carbon disulfide (CS(2)) on pyrogallol-luminol auto-oxidation chemiluminescence (PA-L) system and production of superoxide anion. METHODS: Luminescence dynamic curve of CS(2) and inhibition effect of superoxide dismutase (SOD) on intensity of chemiluminescence induced by CS(2) were studied with PA-L chemiluminescence system. RESULTS: Different concentrations of CS(2) (10, 40, 80 mg/ml) could enhance the emission intensity of chemiluminescence of PA-L system and delayed its peak time, with a significant dose-effect relationship, as compared with that of solvent ethanol. Peak of chemiluminescence induced by 80 mg/ml of CS(2) was higher than its background value without solvent ethanol. Chemiluminescence induced by CS(2) could be inhibited by SOD. CONCLUSION: CS(2) can generate superoxide anion in PA-L system and delay the peak time of chemiluminescence emission dynamic curve. |
| |
| Keywords: | Carbon disulfide Chemiluminescence Pyrogallol Anions Superoxide dismutase |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
