安徽药菊花瓣组织培养技术的研究

目的 :研究安徽药菊花瓣组织培养技术 ,为安徽药菊新品种选育建立最佳条件。方法 :取安徽药菊(滁菊、亳菊、贡菊 )幼蕾花瓣及开放花瓣为外植体 ,按不同方式接种到同种培养基上和按同种方式接种到不同培养基上进行培养 ,诱导其成为完整植株。结果与结论 :所有培养基均能诱导愈伤组织产生 ,但愈伤组织形成和再分化结果有明显差别。愈伤组织诱导花瓣正接优于反接 ,愈伤组织诱导培养基以MS +KT 2mg·L^-1+NAA 0 .2mg·L^-1为宜 ;愈伤分化均以培养基MS +KT 2mg·L^-1+NAA 0 .2mg·L^-1+AgNO3 5mg·L^-1为宜幼蕾花瓣诱导植株再生频率高于开放花瓣 ;所得植株在叶形、及株形等方面发生了变异。

Studies on petal tissue culture of the traditional Chinese medicine Chrysanthemum morifolium

Objective: To set up the optimums for the petal tissue culture of Chrysanthemum morifolium cultivated in Anhui Province . Method: Petals were cut and cultured onto the same kind o f media on different sides and different kinds of media on the same side, and in duced to form the whole plantlet. Result and Conclusion: A ll the media used could induce callus, while the results of callus regeneration was distinct. The MS medium with KT 2 mg·L^-1 and NAA 0.2 mg·L ^-1 w as much better than others when callus were induced. The MS medium with KT 2 mg ·L^-1 +NAA 0.2 mg·L ^-1 and AgNO₃5 mg·L ^-1 was the preferable medium for the bud sprouting. The frequency of plantlets regeneration which was induced by flower bud was higher than that by opening petal. The regenerated pl antlets shod produced variation of leaf and plant shape.