妊娠期高血压疾病患者胎盘组织中溶血磷脂酸受体蛋白Edg4、7的表达及其意义

目的 探讨胎盘组织中溶血磷脂酸受体蛋白Edg4、7的表达与妊娠期高血压疾病发生的关系及作用机制。方法 采用免疫组化链霉菌抗生物素蛋白-过氧化物酶（SP）法,检测20例正常晚期妊娠妇女（正常晚孕组）、20例妊娠期高血压患者（妊娠期高血压组）、20例轻度子痫前期患者（轻度子痫前期组）、30例重度子痫前期患者（重度子痫前期组）的胎盘组织中溶血磷脂酸受体蛋白Edg4与Edg7的表达。结果（1）表达部位：Edg4与Edg7主要表达于胎盘绒毛滋养细胞及蜕膜细胞的细胞质和细胞膜。（2）Edg4和Edg7在胎盘绒毛滋养细胞中的表达阳性率：正常晚孕组分别为25％和20％,妊娠期高血压组分别为60％和40％,轻度子痫前期组分别为80％和65％,重度子痫前期组分别为83．3％和86,7％。轻、重度子痫前期组阳性率明显高于正常晚孕组,两组分别比较,差异有统计学意义（P〈0,05）;妊娠期高血压组与正常晚孕妇组比较,差异无统计学意义（P〉0．05）。（3）Edg4和Edg7在胎盘蜕膜细胞中的表达阳性率：正常晚孕组分别为20％和25％,妊娠期高血压组分别为55％和50％,轻度子痫前期组分别为70％和55％,重度子痫前期组分别为83．3％和73．3％。轻度子痫前期组及重度子痫前期组阳性表达率明显高于正常晚孕组,两组分别比较,差异有统计学意义（P〈0．05）;妊娠期高血压组与正常晚孕组比较,差异无统计学意义（P〉0．05）。结论 妊娠期高血压疾病患者胎盘组织中溶血磷脂酸受体蛋白Edg4、7呈高表达,提示溶血磷脂酸与胎盘组织中的特异性受体Edg4、7结合,并参与了妊娠期高血压疾病的发生。

Expression and significance of Edg4 and Edg7 in the placentas of patients with hypertensive disorder complicating pregnancy

OBJECTIVE: To study Edg4 and Edg7 expression in placenta of women with hypertensive disorder complicating pregnancy, and to investigate the relation between the expression of lysophosphatidic acid (LPA) and hypertensive disorder complicating pregnancy. METHODS: Immunohistochemical SP method was used to measure the expressions of Edg4 and Edg7 in placenta of women with normal pregnancy, 20 women with gestational hypertension, 20 with mild preeclampsia, and with severe preeclampsia. RESULTS: (1) Location: immunohistochemical staining for Edg4 and Edg7 protein were located at the membrane and endochylema of cytotrophoblast as well as decidua cells. (2) The positive expression of Edg4 protein and Edg7 protein on membrane and endochylema of cytotrophoblast was 25% and 20% (normal women), 60% and 40% (gestational hypertension), 80% and 65% (mild preeclampsia), and 83.3% and 86.7% (severe preeclampsia). The expression of Edg4 and Edg7 protein in mild preeclampsia and severe preeclampsia was significantly correlated with the degree of differentiation (P < 0.05). The expression of Edg4 and Edg7 protein showed an insignificant difference in normal pregnant women and gestational hypertension (P > 0.05). (3) The positive expression of Edg4 protein and Edg7 protein on membrane and endochylema of decidua was 20% and 25% (normal pregnancy), 55% and 50% (gestational hypertension), 70% and 55% (mild preeclampsia), and 83.3% and 73.3% (severe preeclampsia) respectively. The expression of Edg4 and Edg7 protein in mild preeclampsia and severe preeclampsia showed a significant correlation with the degree of differentiation (P < 0.05). The expression of Edg4 and Edg7 protein showed an insignificant difference in normal pregnancy and gestational hypertension (P > 0.05). CONCLUSIONS: The high expression of Edg4 and Edg7 protein in the placentas of patients with hypertensive disorder complicating pregnancy indicates that LPA combines with Edg4 and Edg7, inducing the occurrence of hypertensive disorder complicating pregnancy.