BRMS1基因抑制卵巢上皮性癌细胞体内外转移的实验研究

目的 研究BRMS1基因转染卵巢上皮性癌（卵巢癌）细胞后对其体内外转移的抑制作用。方法 应用脂质体介导法将BRMS1基因转染人卵巢癌细胞株A2780细胞内（转染组）,设未进行任何转染的A2780细胞为空白对照组,转染空载体pcDNA3质粒者为阴性对照组。体外观察转染前后3组细胞增殖、凋亡、细胞间缝隙通讯连接、黏附转移情况及超微结构的变化。建立裸鼠人卵巢癌移植瘤模型,观察3组裸鼠BRMS1基因抑制肿瘤体内转移的作用。结果 BRMS1基因成功转染入A2780细胞中。BRMS1基因转染后,空白对照组细胞生长速度与阴性对照组和转染组比较,差异无统计学意义（P〉0．05）;空白对照组、阴性对照组及转染组细胞的每孔克隆数分别为（42±7）、（39±4）及（40±4）个,3组间比较,差异无统计学意义（P〉0．05）;3组细胞S期和G：／M期、Gn／G,期比例比较,差异也无统计学意义（P〉0．05）。转染组细胞问缝隙通讯连接功能增强。细胞侵袭实验显示,转染组转入底层膜的细胞数（112±23）个]明显低于空白对照组和阴性对照组分别为（306±49）、（322±91）个;P〈0．01]。BRMS1基因转染后,裸鼠体内转移灶,转染组为（23±7）个,分别与空白对照组和阴性对照组分别为（96±12）、（112±20）个]比较,差异均有统计学意义（P〈0．01）。结论 作为转移抑制基因,BRMS1基因可抑制卵巢癌细胞的转移。

Inhibitory effect of breast cancer metastasis suppressor l gene on metastasis of human ovarian cancer cell in vitro and in vivo

OBJECTIVE: To study the inhibition effects of breast cancer metastasis suppressor l (BRMS1) gene on metastasis of human ovarian cancer cell in vivo and in vitro. METHODS: BRMS1 gene was transfected into human ovarian cancer cell line A2780 by liposome transfection method. The cells were divided into 3 groups: transfected group with pcDNA3-BRMS1, negative control group with empty plasmid pcDNA3, blank control group without any transfection. Proliferation, apoptosis, growth rate, capability of colony formation, gap junctional intercellular communication (GJIC), capability of adhesion, changes of surface and internal structures of cells were observed in vitro. The cells were injected into athymic mice to establish animal tumor models, and inhibition of the tumorigenicity and metastasis were observed in vivo. RESULTS: BRMS1 gene was transfected into A2780 cell line successfully. There were no significant differences in the growth rate among transfected group, negative control group and blank control group (P > 0.05). The amounts of cell clones per well were 40 +/- 4 in transfected group, 42 +/- 7 in blank control group and 39 +/- 4 in negative control group (P > 0.05 between each two groups). The ratios of S vs G(2)/M and G(0)/G(1) were not significantly different in three groups (P > 0.05). The ultramicrostructure of cells detected by electron microscope showed that GJIC function in transfected group was higher than that in the other two groups. While in migration assay, the numbers of cells in lower chamber passing through the membrane in transfected group, blank control group and negative control group were 112 +/- 23, 306 +/- 49 and 322 +/- 91, respectively; with significant differences among 3 groups (P < 0.01). CONCLUSION: BRMS1 gene could suppress metastasis of ovarian cancer in vitro and in vivo.