| 高效液相色谱法测定复方葛斛胶囊中葛根素含量 |
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| 引用本文： | 杨丽. 高效液相色谱法测定复方葛斛胶囊中葛根素含量[J]. 内蒙古中医药, 2014, 0(5): 53-54 |
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| 作者姓名： | 杨丽 |
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| 作者单位： | 重庆医科大学附属第二医院,400010 |
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| 摘 要： | 目的:测定复方葛斛胶囊中葛根素的含量。方法:采用高效液相色谱法(HPLC法)。色谱柱为C18柱(200mm×4.6mm,5μm),以甲醇-水为流动相,检测波长为250nm。结果:葛根素在0.104~1.04μg范围内线性关系良好,加样回收率为98.73%,RSD为0.69%。结论:HPLC法快速、准确,可用于该制剂的质量控制。
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| 关 键 词： | 葛根素 复方葛斛胶囊 HPLC |
Determination of puerarin in Fufang Gehu Capsules by HPLC |
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| Yang Li. Determination of puerarin in Fufang Gehu Capsules by HPLC[J]. Nei Mongol Journal of Traditional Chinese Medicine, 2014, 0(5): 53-54 |
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| Authors: | Yang Li |
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| Affiliation: | Yang Li(The Second Affiliated Hospital of Chongqing Medical University, Chongqing,China 400010) |
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| Abstract: | Objective：To determine the contents of puerarin in Fufang Gehu Capsules. Methods： HPLC method was adopted and a C18 column （200mm×4.6mm,5μm） was used. The mobile phase was methanol-water.The detection wavelength was at 25Onto. Results： The calibration curve of puerarin was linear in the range of 0.104-1.04μg （r=0.9999）, the averge of recovery was 98.73%, RSD=0.69%. Conclusion： The methods is quick and accurate, can be used for quality control of Fufang Gehu Capsules. |
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| Keywords: | puerarin Fufang Gehu Capsules HPLC |
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