吸入一氧化氮对豚鼠气道阻力的影响

目的:观察持续吸入两种不同浓度NO对豚鼠气道阻力的影响。方法:健康雄性豚鼠36只,随机分为对照组、异丙肾上腺素组、20×10^-6和60×10^-6NO吸入组。用肺功能检测微机处理系统记录各组的基础呼气阻力(R_E)和动态顺应性(C_dyn),以及每次静脉给予组胺后R_E和C_dyn的改变。结果:①静脉给予80、120及160μg/kg组胺后,20×10^-6及60×10^-6NO吸入组的R_E值明显低于对照组;给予较低浓度组胺(20、40μg/kg)后,吸入NO两浓度组的R_E值也低于对照组,但无显著差异。②给予80、120、160μg/kg组胺后,NO吸入组的C_dyn明显高于对照组;在较低浓度组胺激发后,吸入NO两组的C_dyn改变与对照相比亦无明显差异。③在给予80、120及160μg/kg组胺后,吸入NO两组的R_E值显著高于异丙肾组,而C_dyn值则显著低于异丙肾组。④各个阶段吸入NO两浓度组的R_E及C_dyn值均无明显差别。结论:在高浓度组胺激发后,吸入NO对组胺的拮抗作用较为显著,但与异丙肾相比较弱,20×10^-6和60×10^-6NO的拮抗作用没有强度上的差别。

Effects of inhaled nitric oxide on airway resistance in guinea pigs

AIM:To observe the effect of continuous inhaled nitric oxide (NO) on airway resistance in guinea pigs. METHODS:36 healthy male guinea pigs were divided into control group, isoproterenol group and two inhaled NO (20×10^-6 and 60×10^-6) groups. Respiratory resistance (R_E) and dynamic compliance(C_dyn)were recorded before and after evoked by histamine at different doses. RESULTS:After injections of intravenous histamine at 80,120 and 160 μg/kg, the R_E of inhaled NO groups were apparently lower than that of control group. Compared with control group, the C_dyn of inhaled NO groups were significantly higher after administration of histamine at 80, 120 and 160 μg/kg. After given histamine at more than 80 μg/kg,the R_E of inhaled NO groups were higher and the C_dyn lower than those of isoproterenol group. CONCLUSION:Inhalation of 20×10^-6NO and 60×10^-6NO can inhibit the increase in airway resistance induced by higher doses (80,120 and 160 μg/kg )of intravenous histamine, but the effect of intravenous isoproterenol seems stronger.