| 紫杉醇诱导人食管癌细胞凋亡的实验研究 |
| |
| 引用本文： | 侯俊民. 紫杉醇诱导人食管癌细胞凋亡的实验研究[J]. 浙江中西医结合杂志, 2011, 21(12): 849-850 |
| |
| 作者姓名： | 侯俊民 |
| |
| 作者单位： | 江苏省泰州市人民医院,泰州,225300 |
| |
| 摘 要： | 目的：研究紫杉醇诱导人食管癌细胞的凋亡作用，并探讨其诱导凋亡的分子机制。方法：通过形态学观察，流式细胞仪技术分析研究紫杉醇对人食管癌细胞的凋亡作用，免疫组化测定bcl-2、p53、p21在紫杉醇处理前后的变化。结果：紫杉醇作用于Eea109细胞后，可见到较典型的细胞凋亡形态学变化：细胞核固缩、解聚以及凋亡小体。流式细胞仪检测显示，G1峰前有一明显的凋亡峰。免疫组化显示，bcl-2、p53在紫杉醇处理前后无变化，而p21表达增加。结论：紫杉醇可能通过p21诱导人食管癌细胞凋亡。
|
| 关 键 词： | 食管癌细胞 细胞凋亡 紫杉醇 |
Apoptosis in Human Esophageal Carcinoma Cells Induced by Paclitaxel in vitro |
| |
| Hou Junmin. Apoptosis in Human Esophageal Carcinoma Cells Induced by Paclitaxel in vitro[J]. Zhejiang Journal of Integrated Traditional Chinese and Western Medicine, 2011, 21(12): 849-850 |
| |
| Authors: | Hou Junmin |
| |
| Affiliation: | Hou Junmin.Taizhou People's Hospital,Taizhou(225300),China |
| |
| Abstract: | Objective:To study the effect of paclitaxel on inhibiting human eophageal carcinoma cells and the molecular mechanism of the apoptosis.Methods:The apoptosis of esophageal carcinoma cells and the changes of bcl-2,p53,and p21 were observed morphologically and were analyzed with flow-type cytometry after the treatment with paclitaxel. Results:Typical morphologic changes of apoptosis such as nucleus pyknois,nucleus depolymerization,and apoptic bodies, were found after paclitaxel acting on Ecal09 cells.Flow-type... |
| |
| Keywords: | apoptosis esophageal carcinoma cell paclitaxel |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
