| 硫酸-甲醇法测定劲健胶囊中人参总皂苷含量 |
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| 引用本文： | 方平美,何朋朋. 硫酸-甲醇法测定劲健胶囊中人参总皂苷含量[J]. 浙江中医药大学学报, 2011, 0(5): 747-749,752 |
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| 作者姓名： | 方平美 何朋朋 |
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| 作者单位： | 浙江省杭州天诚药业有限公司 杭州 311231 |
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| 摘 要： | [目的]研究用硫酸-甲醇法测定劲健胶囊中人参总皂苷的含量。[方法]将样品用甲醇提取,经Ambrlite-XAD-2大孔树脂和中性氧化铝纯化,以人参皂苷Re为对照品,浓硫酸-甲醇显色,在321nm处测定总皂苷含量。[结果]在21.0～126.0μg吸光度与含量成良好线形关系r=0.9999(n=6),含量的加样回收率为95.1%,RSD1.1%(n=9)。[结论]硫酸-甲醇法比香草醛-高氯酸-冰醋酸法操作简便、快速、灵敏、重复性好,可以作为总皂苷质量评价的定量分析方法。
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| 关 键 词： | 劲健胶囊 人参总皂苷 硫酸-甲醇法 功效成份测定 |
Ginseng Total Saponin Content Measured with Vitriol-carbinol in Jinjian Capsule |
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| Fang Pingmei,He Pengpeng. Ginseng Total Saponin Content Measured with Vitriol-carbinol in Jinjian Capsule[J]. Journal of Zhejiang University of Traditional Chinese Medicine, 2011, 0(5): 747-749,752 |
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| Authors: | Fang Pingmei He Pengpeng |
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| Affiliation: | Fang Pingmei,He Pengpeng Hangzhou Tiancheng Pharmaceutical Co.Ltd.,Zhejiang Province(311231) |
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| Abstract: | [Objective]To measure the content of total ginseng saponin in Jinjian Capsule with vitriol-carbinol method.[Method]Extract carbinol from sample,by Ambrlite-XAD-2 macroporous resin and neutral alumina purification,take saponin Re as control,developing with concentrated sulfuric acid-carbinol,measure total saponin at 321nm.[Result]At 21.0~126.0μg,the absorbance and content has good linear relation,r=0.9999(n=6),the sample recovery rate is 95.1%,RSDl.l%(n=9).[Conclusion]Vitriol-carbinol method is simple to operate,quick,sensitive,with good repeatability, and can be a quantitative analysis for quality evaluation to total saponin. |
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| Keywords: | Jinjian Capsule total ginseng saponin vitriol-carbinol method measure functional component |
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