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A fixation-decalcification procedure for bone biopsies
Authors:G BUSSOLATI
Institution:Istituto di Anatomia e Istologia Patologica, Universitàdi Torino, Via Santena;7, 10126 Turin, Italy
Abstract:A procedure for the simultaneous fixation and decalcification of bone biopsies is described. Mild acidic fixatives (such as Bouin's fluid) are effective decalcifying agents when made calcium-free by a continuous circulation through a column of anion-binding ion exchange resin. The procedure has been found relatively fast, safe, economic and to provide excellent histology of biopsies containing cancellous bone.
Keywords:bone decalcification  bone fixation  bone biopsies
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