肺癌组织中促凋亡基因Bax的表达及意义

为了探讨促凋亡基因Bax有肺癌中的表达及意义，采用免疫组化方法对113例肺癌标本中Bax的表达情况进行检测分析，结果：113例肺部Bax表达的阳性率为60．2％，（68／113），其中鳞癌61．2％（30／49），腺癌57．8％（26／45），腺鳞癌50．0％（3／6），大细胞癌71．4％（5／7），小细胞癌66．7％（4／6），Bax阳性率在非小细胞肺癌（59．85）与小细胞肺癌（66．7％）之间无差异的显著性（P＞0．05），与病人年龄、性别无明显关系（P＞0．05），与组织的分化程度及淋巴结转移有关（P＜0．01及P＜0．05）。结果表明，促凋亡基因Bax在肺癌组织中具有较高的表达，说明其参与了肺癌细胞凋门的调节，并与肿瘤的分化程度及淋巴结转移有关。

Expression of Proapoptotic Gene Bax in Lung Carcinoma and Its Significance

Objective: to study the expression of proapoptotic gene Bax in lung carcinoma and its significance Method: immunohistochemical staining with a rabbit anti-human polyclonal antibody Bax was carried out on 113 paraffin-embedded lung carcinoma samples Results: the positive rate of Bax protein was 60 2%(68/113) including 30 of 49 squamous cell carcinomas(61 2%), 26 of 45 adenocarcinomas(57 8%), 3 of 6 small cell carcinomas (50 0%), 5 of 7 large cell carcinomas (71 4) and 4 of 6 small cell carcinomas(66 7%) respectively There was no significant difference between NSCLC and SCLC(P>0 05) The expression of Bax protein was not correlated to age, sex (P>0 05), but strongly associated with the differentiation degree of the tumor and lymph node metastasis (P<0 01 and P<0 05). Conclusion: proapoptotic gene Bax which is overexpressed in lung carcinoma may take part in the modulation of apoptosis and is associated with the differentiation of lung carcinoma and lymph node metastasis.