胶州湾菲律宾蛤仔甲型肝炎病毒污染调查及对策研究

为查明胶州湾菲律宾蛤受ＨＡＶ污染情况，于１９９１．７～１９９２．８月间，在湾内设６个监测点，每月定时、定点采集标本共７５份。经核酸斑点杂交试验，ＨＡＶ分离及ＰＣＲ法检测ＨＡＶ前体ＲＮＡ，结果表明：胶州湾的一定海域，一定时间的菲律宾蛤有ＨＡＶ污染迹象，但已失去生物活性。同时流行病学调查证实，菲律宾蛤不是青岛市区甲型肝炎（甲肝）发病和流行的直接原因。但鉴于市民生活污水直接排入胶州湾内，必须加强市区生活污水治理和对市民食用海产品的饮食卫生教育。

Study on the Contamination of Ruditapes Philippinarum by HAV in Jiaozhou Bay and Its Countermeasures

Six surveillance spots were set up in Jiaozhou Bay to examine the contamination of RuditapesPhilippinarum(R. P)by HAV. Specimens were collected regularly every month.Seventy-fivespecimens were collected in July 1991- Aug 1992.The precursor RNA of HAV was detected bymethods of PCR,RNA dot-blot hybridization and HAV isolation. It showed that R.P. had beencontaminated by HAV in some areas of the bay at times,but the virus was in a state of inactivity.Epidemiologic investigation in the same period proved that R.P. was not the direct cause of HAepidemic in Qingdao. However,the domestic sewage is directly drained into the Jiao zhou Bay sothe control of the city sewage drainage system must be enforced and health education serve forpeople in taking sea food and drink should be strengthened.