雷公藤甲素对瘢痕疙瘩成纤维细胞增殖和胶原合成的影响

目的探讨雷公藤甲素对瘢痕疙瘩成纤维细胞增殖和胶原合成的影响。方法取手术切除的瘢痕疙瘩组织作成纤维细胞的原代培养,传代培养后加入不同浓度的雷公藤甲素作用,观察细胞的形态学变化,应用MTT法检测细胞的活性,应用免疫组化和Western-blot法检测雷公藤甲素对瘢痕疙瘩成纤维细胞Ⅰ型、Ⅲ型前胶原合成的影响。结果雷公藤甲素能显著抑制瘢痕疙瘩成纤维细胞的增殖,且存在一定的浓度依赖性;雷公藤甲素作用后瘢痕疙瘩成纤维细胞Ⅰ型、Ⅲ型前胶原表达减弱。结论雷公藤甲素能有效抑制瘢痕疙瘩成纤维细胞的增殖和Ⅰ型、Ⅲ型前胶原的合成。

Effect of Triptolide on the Proliferation of Fibroblast and Synthesis of Collagen in Keloid

Objective To investigate the effect of triptolide on the proliferation of fibroblast and synthesis of collagen in keloid.Methods Tissue samples from patients with keloid were harvested for in vitro culture of fibroblasts,which were treated with triptolide in different concentrations in exponential phase of cell growth.The morphology of the cells was observed after treatment.Cell activity was determined by MTT.The expression of procollagen typeⅠand Ⅲ in vitro after medication were identified by immunohistochemistry and Western blotting analysis. Results It was found that triptolide could significantly inhibit the growth of fibroblasts in vitro in a concentration-dependent manner.Further the expression of procollagen typeⅠand Ⅲ in vitro were decreased after triptolide action. Conclusion Triptolide can effectly inhibit the growth of fibroblasts and the production of procollagen typeⅠand Ⅲ of keloid in vitro.