| 高效液相色谱法测定鬼臼毒素酊的含量 |
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| 引用本文： | 谢冬梅,刘斐. 高效液相色谱法测定鬼臼毒素酊的含量[J]. 中国药业, 2010, 19(21): 18-19 |
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| 作者姓名： | 谢冬梅 刘斐 |
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| 作者单位： | 1. 蚌埠医学院第一附属医院药剂科,安徽,蚌埠,233004
2. 合肥远志医药科技开发有限公司,安徽,合肥,230028 |
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| 摘 要： | 目的采用高效液相色谱法测定鬼臼毒素酊中鬼臼毒素的含量。方法色谱柱为Shimpack VP-ODS柱（150mm×4.6mm,5μm）,流动相为甲醇-水（63：37）,流速1.0mL/min,检测波长292nm。结果鬼臼毒素质量浓度在0.2012～0.8048μg/mL范围内与峰面积线性关系良好（r=0.99993）,平均回收率为99.68%,RSD=0.48%（n=6）。结论高效液相色谱法简便、准确、快速,可有效控制鬼臼毒素酊中鬼臼毒素的含量。
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| 关 键 词： | 鬼臼毒素酊 鬼臼毒素 高效液相色谱法 含量 |
Content Determination of Podophyllotoxin Tincture by HPLC |
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| Xie Dongmei,Liu Fei. Content Determination of Podophyllotoxin Tincture by HPLC[J]. China Pharmaceuticals, 2010, 19(21): 18-19 |
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| Authors: | Xie Dongmei Liu Fei |
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| Affiliation: | 1.Department of Pharmacy,First Affiliated Hospital,Bengbu Medical College,Bengbu,Anhui,China 233004;2.Hefei Yuanzhi Pharmaceutical R ＆ D Co.,Ltd.,Hefei,Anhui,China 230038) |
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| Abstract: | Objective To establish an HPLC method for the determination of podophyllotoxin in Podophyllotoxin Tincture.Methods The analytical column was C18 column（150 mm × 4.6 mm,5 μm） and the mobile phase was methanol-water（63：37） at the flow rate of 1.0 mL /min.The UV detection wavelength was at 292 nm.Results The linearity was good for podophyllotoxin within the range of 0.201 2-0.804 8 μg /mL（r = 0.999 93）,the mean recovery rate was 99.68%（RSD = 0.48%,n = 6）.Conclusion The method is simple,rapid and accurate,which is effective to control the podophyllotoxin content in Podophyllotoxin Tincture. |
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| Keywords: | Podophyllotoxin Tincture podophyllotoxin HPLC content |
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