血管内皮生长因子C受体3mRNA在喉鳞状细胞癌中的表达

目的探讨喉鳞状细胞癌组织与细胞中血管内皮生长因子(VEGF)-C、受体3(R-3)mRNA的表达与颈淋巴转移的关系,以期有助于喉鳞癌生长和转移机制的进一步阐明。方法用原位杂交方法检测24例喉鳞癌组织和5例癌旁正常黏膜VEGF-C、VEGFR-3 mRNA的表达,进行比较,并按年龄、T分期、病理分级、淋巴转移进行分组,分别探讨三者与各临床病理参数及淋巴转移的关系。结果原位杂交方法显示:VEGF-C、VEGFR-3 mRNA在喉鳞状细胞癌组织的阳性表达明显高于癌旁正常黏膜;VEGF-C、VEGFR-3 mRNA与患者年龄、T分期、病理分级均无关。同时显示VEGF-C、VEGFR-3 mRNA在淋巴转移组中的阳性表达明显高于未转移组。结论VEGF-C、VEGFR-3 mRNA在喉鳞状细胞癌组织及细胞中的表达增高,可能是喉癌发生发展的原因之一。

The expressions of vascular endothelial growth factor C and receptor-3 mRNA in laryngeal squamous cell carcinoma

Objective To explore the expressions of vascular endothelial growth factor C(VGEF-C) and receptor-3 mRNA in laryngeal squamous cell carcinoma(LSCC) correlated to lymph node invasion of the neck,and add to understanding of mechanisms underlying growth and metastasis of laryngeal carcinoma.Methods VEGF-C and VEGFR-3 mRNA in 24 LSCC samples and 5 samples collected from tumor-adjacent normal mucosa were detected by in-situ hybridization techniques.Data obtained were classified based on age,TMN staging,pathological grading and lymphatic metastasis.Correlations of age,TNM staging and pathological grading with varied pathological findings and presence of lymphatic metastasis were analyzed,respectively.Results In-situ hybridization showed higher levels of VEGF-C and VEGFR-3 mRNA in LSCC vs tumor-adjacent normal tissue.Expressions of VEGF-C and VEGFR-3 mRNA were not found to be correlated with age,TNM staging and pathological grading.In addition,higher levels of VEGF-C and VEGFR-3 mRNA were detected in patients with vs without tumor metastasis.Conclusion Enhanced expressions of VEGF-C and VEGFR-3 mRNA were shown in LSCC and may be one of factors underlying oncogenesis.