预防使用大剂量甲基强的松龙对大鼠急性脊髓损伤的神经保护作用

目的研究预防使用大剂量甲基强的松龙对急性脊髓损伤大鼠的神经功能保护作用方法采用Allen重物打击模型，动物随机分为三组：对照组；脊髓损伤组；预防使用大剂量甲基强的松龙组。分别在脊髓损伤后24h、72h进行神经功能评分(Tarlov评分障碍率、Molt斜板功能障碍率)、脊髓病理形态学及超微结构观察、神经中丝(NF)及胶质纤维酸性蛋白(GFAP)观察、结果预防使用大剂量MP可明显改善损伤脊髓的病理形态及超微结构；脊髓损伤后72h大鼠神经功能评分明显提高；显著提高NF的表达、抑制GFAP的表达．结论预防使用大剂量甲基强的松龙对大鼠急性脊髓损伤有神经保护作用

Neuroprotective effect of high-dose methylprednisolone in prophylaxis in rat acute spinal cord injury

Objective To study the neuroprotective effect of high-dose methylprednisolone in prophylaxis in rat acute spinal cord injury. Methods The rats were randomly divided into three groups:Control groups,acute spinal cord injury groups,administration of methylprednisolone in prophylaxis. Allen's weight drop model of acute spinal cord injury was prepared and to study the ratio of Tarlov's motor scales,ratio of Molt's inclined plane scales,pathological and ultrastructural changes of spinal cord,neurofilament and glial fibrillary acidic protein at 24- and 72-hour after ASCI,respectively. Results The pathological and ultrastructural changes of spinal cord were significantly improved in MP groups. The neurological function of rats in MP groups was significantly improved at 72-hour after ASCI. The expression of NF significantly increased and the expression of GFAP significantly decreased in MP groups. Conclusion There is neuroprotective effect of high-dose methylprednisolone in prophylaxis in rat acute spinal cord injury.