复方薤白胶囊对野百合碱诱导肺动脉高压大鼠血浆中花生四烯酸代谢产物的影响

目的探讨复方薤白胶囊(CMOC)对野百合碱(MCT)诱导大鼠肺动脉高压中花生四烯酸代谢产物的影响。方法将SD大鼠60只,借助SAS统计分析系统PROCPLAN程序进行随机分组共分为正常对照组、模型组、卡托普利组、CMOC大剂量组和CMOC小剂量组5组。采用腹腔注射MCT的方法制作动物模型,测定其平均肺动脉压和平均右心室压,采用放射免疫法进行血浆中血栓烷素B2(TXB2)、6酮前列腺素Fia(6KPGFia)测定。结果平均肺动脉压正常对照组为(16.4±2.1)mmHg(1mmHg=0.133kPa),模型组为(36.9±4.8)mmHg,两组比较差异有统计学意义(P<0.01);CMOC大、小剂量组则分别为(26.2±2.8)、(27.9±2.8)mmHg,与模型组比较差异均有统计学意义(P均<0.01),CMOC大、小剂量组降低平均肺动脉压作用优于卡托普利组。模型组与正常对照组比较血浆中TXB2明显增高(P<0.05),而6KPGFia含量显著下降(P<0.01),CMOC大剂量组与模型组相比血浆中TXB2明显下降,6KPGFia含量显著上升,差异均有统计学意义(P<0.05,<0.01)。结论CMOC能有效地降低大鼠肺动脉高压,其作用原理可能是通过降低血浆中TXB2的含量,增加6KPGFia的分泌。

The effect of compound macrostem onion capsule on metabolism of arachidonic acid in a rat model of monocrotaline-induced pulmonary artery hypertension

OBJECTIVE: To study the effect of compound macrostem onion capsule (CMOC) on products of arachidonic acid metabolism in monocrotaline (MCT)-induced pulmonary artery hypertension rats. METHODS: Sixty SD rats were divided into a control group, a model group, and three experimental groups in which the rats were fed with captopril, small dose single dosage, and large dose (double dosage) of CMOC, respectively. The rat model was made by MCT peritoneal injection. The mean pulmonary arterial pressure (mPAP) and the mean right ventricular pressure (mRVP) were measured. Thromboxane B(2) (TXB(2)) and 6-keto-prostaglandin Fia (6-K-PGFia) were determined by RIA method. RESULTS: The mPAP in rats of the model group (36.9 +/- 4.8) mm Hg, 1 mm Hg = 0.133 kPa] was significantly higher than that of the control group (16.4 +/- 2.1) mm Hg, all P < 0.01]. The mPAP in rats of the double dosage and single dosage of CMOC groups were (26.2 +/- 2.8) mm Hg and (27.9 +/- 2.8) mm Hg, respectively; both were significantly lower than those of the model group (all P < 0.01). TXB(2) in rats of the model group was significantly higher than that of the control group (P < 0.05), while 6-K-PGFia in the model group was significantly lower than that of the control group (P < 0.01). TXB(2) in rats of the double dosage CMOC group was significantly lower than that of the model group, but 6-K-PGFia in rats of the CMOC groups was significantly higher than that of the model group (P < 0.05, < 0.01). CONCLUSION: CMOC is effective in reducing pulmonary artery hypertension. Inhibition of TXB(2) and augmentation of 6-K-PGFia might be the underlying mechanisms.