放射性碘致大鼠甲状腺损伤超微结构的形态计量学研究

本文应用透射电镜观察了在较低剂量１３１Ｉ作用大鼠甲状腺滤泡上皮细胞超微结构的变化特点，分析了线粒体的变异发生率，并应四川大学研制的ＭＩＡＳ－３００图像分析系统和用形态计量学方法，测定了胞质内粗面内质网的二维和三维形态计量学参数。结果表明：所测的各项参数都有随剂量增高而增大的趋势，并呈现较好的效应剂量依赖关系。由此计算的Ｄ２５值和Ｄ５０值，以粗面内质网体积密度的变化最为明显。提示，低剂量１３１Ｉ作用于滤泡上皮细胞，其早期应重视内质网的损伤效应，以及由此导致合成类固醇激素和外源性蛋白质的障碍

A morphometric study of the ultrastructure changes due to 131 I-induced injury in rat thyroid

A morphometric study of the ultrastructure changes induced by 131 I injury in rat thyroid was performed by using the electron microscope. The incidence of abnormal mitochodria was analysed. The morphometric parameters of celluar rough endoplasmic reticulum (RER) including two and three-dimensions were measured under image analysis techniques and morphometric methods. The results showed that the values of the morphometric parameters were gradually increased with the increasing of 131 I doses and in a good dose-effect relationship. Especially, the increase of RER volume density was more promirent than the others. Our study indicates that in the early stage, more attention should be paid to the RER injury under lesser 131 I doses, because it may impair the synthesis of the steroid hermone and the exogenic protein.