Evaluation of antioxidant properties of a new compound, pyrogallol-phloroglucinol-6,6'-bieckol isolated from brown algae, Ecklonia cava |
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| Authors: | Sung-Myung Kang Seung-Hong Lee Soo-Jin Heo Kil-Nam Kim You-Jin Jeon |
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| Institution: | 1Department of Marine Life Science, Jeju National University, 1 Ara 1-dong, Jejudaehak-ro, Jeju 690-756, Korea.;2Marine Living Resources Research Department, Korea Ocean Research and Development Institute, Ansan 426-744, Korea.;3Korea Basic Science Institute (KBSI), Jeju 690-140, Korea. |
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| Abstract: | In this study, antioxidant and free radical scavenging activities of the natural antioxidative compound, pyrogallol-phloroglucinol-6,6''-bieckol (PPB) isolated from brown algae, Ecklonia cava was assessed in vitro by measuring the radical scavenging activities (DPPH, alkyl, hydroxyl, and superoxide) using electron spin resonance (ESR) spectrometry, intracellular reactive oxygen species (ROS) scavenging activity, and DNA damage assay. According to the results of these experiments, the scavenging activity PPB against difference radicals was in the following order: DPPH, alkyl, hydroxyl, and superoxide radicals (IC50; 0.90, 2.54, 62.93 and 109.05 µM). The antioxidant activities of PPB were higher than that of the commercial antioxidant, ascorbic acid. Furthermore, PPB effectively inhibited DNA damage induced by H2O2. These results suggest that the natural antioxidative compound, PPB, can be used by the natural food industry. |
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| Keywords: | Ecklonia cava brown algae pyrogallol-phloroglucinol-6 6''-bieckol antioxidant |
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