Abstract: | Objective To comprehend antibiotic resistance and MLST (multilocus sequence typing) typing characteristics of clinical isolates of Klebsiella pneumoniae in Guangdong province. Methods 102 strains of Klebsiella pneumoniae were collected from nine cities in Guangdong province. The sensitivities of the strains to 30 antibacterial drugs were determined by the broth dilution method. In addition, all isolated strains were classified into MLST, and their distribution characteristics were analyzed. Results According to the results of drug sensitivity tests and string tests, the drug resistance of 102 strains of clinical isolated Klebsiella pneumoniae from nine cities of Guangdong province could be divided into four types, namely, (ESBLs) KP (43strains, 9 of which were ESBLs-hvKP), CRKP (8 strains, 1 of which was CR-hvKP), more sensitive KP (30 strains, all were hvKP), and other types of multidrug-resistant KP (MDRKP) (21 strains, 6 of which were MDR-hvKP). Results of MLST classification indicated that 102 clinical isolates could be divided into 54 MLST types, among which six were newly discovered ST types. In terms of regional distribution, Zhanjiang was dominated by ST23 (4/30); Maoming was dominated by ST1224 (4/12); Yangjiang was dominated by ST11 (3/6); Zhuhai was dominated by ST15 (3/8), and the ST types in other areas were polymorphic. Conclusion The drug resistance of clinical isolates of Klebsiella pneumoniae from our province was serious, and the distribution of bacterial flora was diverse. There were some variation strains in some areas, and thus the monitoring of drug resistance and the change of microflora structure should be |