年龄和性别对正常人外周血淋巴细胞核质桥自发率的影响

目的： 探索年龄、性别因素对我国健康人群外周血淋巴细胞核质桥自发率的影响，为核质桥的影响因素研究提供科学依据。方法： 选取98例健康成人，其中男性52例，女性46例，年龄范围为20~68岁，平均年龄为（40.7±12.6）岁。按年龄分为20~29岁组（22人）、30~39岁组（23人）、40~49岁组（25人）和50岁以上组（28人）。抽取研究对象外周血2 mL，采用胞质分裂阻滞法培养细胞40 h后，加入松胞素B （终浓度为10 μg/mL），继续培养至68 h收获细胞。每个受试者观察1 000个双核细胞，分析核质桥、微核及核芽的数量。结果： 核质桥总体自发率为每个细胞0.56‰，男性略高于女性，但差异无统计学意义（P > 0.05）。男性在不同年龄组间，核质桥自发率差异无统计学意义（P > 0.05）。女性40~49岁年龄组核质桥自发率最高，显著高于20~29岁组（U=2.31，P < 0.05）。微核自发率女性高于男性（U=4.40，P < 0.05），男性和女性受试者40~49岁组微核自发率均为最高。核芽自发率在不同性别间的差异无统计学意义（P > 0.05），在不同年龄组间无明显变化规律。结论： 健康人群中的核质桥自发率较低，不受性别因素影响，在20~49岁范围内具有随年龄增加而升高的趋势。

Effects of age and gender on formation of nucleoplasmic bridges in peripheral blood lymphocytes

OBJECTIVE: To investigate influence of age and gender on spontaneous formation of nucleoplasmic bridges (NPB) in peripheral blood lymphocyte from in healthy Chinese subjects. METHODS: A total of 98 healthy adults (52 male and 46 female) aged 2068 years (40.7 ±12.6 years) were enrolled. The subjects were divided into different age groups:20-29 (22 subjects), 30-39 (23 subjects), 40-49 (25 subjects) and over 50 (28 subjects). At 40 h of their lymphocyte cultures in the cytokinesis-block cytome assay, cytochalasin-B (10 μg/mL) was added and cultures were harvested at 68 h. The presence of NPB, micronucleus (MN) and nuclear bud (NBD) in their peripheral blood lymphocytes were determined by analyzing 1 000 binucleated cells in each sample. RESULTS: The overall NPB spontaneous rate was 0.56‰, but the rates were non-significantly higher in males than in females. In addition, there was no statistically significant difference in male subjects among different age groups. However, the highest NPB spontaneous rate in females was found in 40-49 age group which was significantly higher than that in the 20-29 age group (U=2.31, P < 0.05). The MN spontaneous rates in females were higher than that in males (U=4.40, P < 0.05) and the highest rates in both male and female subjects were found in the 40-49 age groups. There was no statistical difference of NBD spontaneous rates between genders and among different age groups. CONCLUSION: NPB spontaneous rate in healthy population was very low which was not affected by gender but had increased trend within the 40-49 age group.