2型糖尿病对小鼠下颌骨骨再生及辅助性T细胞17、调节性T细胞相关因子表达的影响

目的观察2型糖尿病对小鼠下颌骨骨再生以及辅助性T细胞17 (Th17)、调节性T细胞(Treg)相关因子表达的影响。方法将36只6周龄C57BL/6J雄性小鼠随机分为正常对照(NC)组和2型糖尿病(T2DM)组,小鼠下颌骨缺损建模当天(0 d)和术后7、14、28 d检测空腹血糖。于两组小鼠下颌骨缺损建模后7、14、28 d,通过苏木素-伊红(HE)染色观察下颌骨缺损愈合情况,通过免疫组织化学染色的方法观察下颌骨缺损内碱性磷酸酶(ALP)、Runt相关转录因子2 (RUNX2)、叉头框蛋白P3 (Foxp3)、维甲酸相关孤核受体γt(RORγt)和蛋白酪氨酸磷酸酶非受体型2 (PTPN2)的表达情况。结果 HE染色结果显示:T2DM组小鼠的下颌骨缺损范围内新生骨的数量明显少于NC组。免疫组织化学染色结果显示:成骨相关蛋白ALP和RUNX2的表达在T2DM组的骨缺损内明显降低;另外,2型糖尿病小鼠下颌骨缺损范围内的RORγt阳性细胞数量增加,Foxp3阳性细胞数量减少,PTPN2的表达明显降低。结论 2型糖尿病可以显著地抑制小鼠下颌骨骨再生过程,PTPN2的下降以及Treg-Th17向偏...

Finite element analysis of the comprehensive impact of scar and maxillary expansion combined with protraction on the development of maxilla with cleft lip and palate after repair operation

Objective To study the comprehensive impact of scar and maxillary expansion combined with protraction on the development of maxilla with cleft lip and palate after repair operation. Methods In the original finite element model of the maxilla with cleft palate, a finite element model of the maxilla with cleft lip and palate was established by using Boolean operation in ANSYS. Scar force after cleft lip and palate repair and maxillary expansion force combined with protraction were added simultaneously to process the stress analysis. Results Maxillary deformation occurred in the three-dimensional direction. The comparison of displacements was as follows: X-axis>Z-axis>Y-axis. Conclusion Maxillary growth is significantly inhibited in the three-dimensional direction under the comprehensive impact of scar and maxillary expansion combined with protraction after repair operation, especially transverse and sagittal growth.