细粒棘球蚴囊液蛋白组分分析

目的 利用鸟枪法分析细粒棘球蚴(Echinococcus granulosus)囊液蛋白(hydatid cyst fluid protein,HCFP)组分,寻找其中具有潜在调控免疫失调性疾病功能的活性组分.方法 无菌收集细粒棘球蚴病患者肝囊肿中的细粒棘球蚴囊液,采用鸟枪法进行液相色谱-串联质谱鉴定,采用MaxQua...

Prevalence of soil-borne nematode infections among residents living in urban/town areas of China in 2015

Objective　To analyze the components of proteins from Echinococcus granulosus cyst fluid using the shotgun method, and to identify the active components with potential regulatory effects for immune dysregulation diseases. Methods　The E. granulosus cyst fluid was collected aseptically from the hepatic cysts of patients with cystic echinococcosis, and characterized by liquid chromatography （LC） tandem mass spectrometry （MS/MS） following digestion with trypsin. The protein data were searched using the software MaxQuant version 1.6.1.0 and the cellular components, molecular functions, and biological processes of the identified proteins were analyzed using the Gene Ontology （GO） method. Results　The E. granulosus cyst fluid separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis （SDS⁃PAGE） had a relative molecular mass of 25 to 70 kDa. LS⁃MS/MS analysis identified 37 proteins, including 32 known proteins and 5 unknown proteins. At least 4 proteins were preliminarily found to exhibit potential regulatory effects for immune dysregulation diseases, including antigen B, glutathione⁃S⁃transferase （GST）, thioredoxin peroxidase （TPX） and malate dehydrogenase （MDH）. GO enrichment analysis showed that the identifiedproteins had 149 molecular functions and were involved in 341 biological processes. Conclusions 　E. granulosus cyst fluid has a variety of protein components, and four known proteins are preliminarily identified to be associated with immune dysregulation diseases.