利用山茶油提高刺糖多孢菌AEG3-1发酵多杀菌素生产

目的 以诱变菌株Saccharopolyspora spinosa AEG3-1为试验株，研究山茶油对该菌株发酵生产多杀菌素的影响。方法 首先单因素实验筛选到最佳植物油脂，再利用响应面实验设计对发酵培养基中最佳植物油脂、葡萄糖、糊精、棉籽蛋白4种成分进行优化，最后在5L罐进行验证。结果 最佳植物油脂是山茶油，在0h添加15.0g/L效果最好。在此基础上，获得优化发酵培养基(g/L)：山茶油15.1、葡萄糖51.6、糊精19.73、棉籽蛋白21.8，其产量达318.25mg/L，提高了55.61%。在5L罐中，利用优化培养基，分批和补料发酵产量分别达到361.97和512.36mg/L。结论 通过添加山茶油，诱变株的多杀菌素产量显著提高，说明该生产工艺确实可行，可为多杀菌素大规模生产提供可靠的依据。

Enhancement of spinosad production by Saccharopolyspora spinosa #br# AEG3-1 using camellia oil

Objective The effect of camellia oil on spinosad production by Saccharopolyspora spinosa AEG3-1 was studied. Methods Firstly, the optimum vegetable oil was selected by the single factor experiment. Then, the concentrations of the optimal vegetable oil, glucose, dextrin, and cottonseed protein in the fermentation medium were optimized by using the response surface methodology design. Finally, results were verified in a 5L fermenter. Results The optimum vegetable oil was camellia oil of 15.0g/L at 0h. The optimum medium (g/L) was as follows: Camellia oil 15.1, glucose 51.6, dextrin 19.7, cottonseed protein 21.8, and the production reached 318.25mg/L with an increase of 55.61%. In a 5L fermenter, using the optimized media, the spinosad production in batch and fed-batch fermentation reached 361.97mg/L and 512.36mg/L, respectively. Conclusion The spinosad production of the mutant strain was significantly improved by adding camellia oil, indicating that the production process was feasible and could provide a reliable basis for the large-scale production of