鼻息肉中一氧化氮合酶表达及其意义

目的 :了解鼻息肉 (NP)中一氧化氮合酶 (NOS)的分布特点和活性及其在NP发病中的作用。方法 :用免疫组织化学法检测 30例NP(NP组 )及 2 0例正常鼻黏膜 (正常鼻黏膜组 )中NOS的表达 ,并用分光光度计法检测其活性。结果 :NP组NOS活性为 (4.0 79± 0 .6 5 5 )U/mg蛋白 ,正常鼻黏膜组为 (1.5 2 6± 0 .310 )U/mg蛋白 ,二者间差异有统计学意义 (P <0 .0 1) ;NP组iNOS有大量细胞表达 ,分布在黏膜上皮、腺体和血管内皮细胞以及炎症细胞中 ,与正常鼻黏膜组比较 ,差异有统计学意义 (P <0 .0 1) ;而eNOS也有表达 ,但与正常鼻黏膜组比较 ,差异无统计学意义 ;NP组i NOS表达明显高于eNOS表达 ,其差异有统计学意义 (P <0 .0 1)。结论 :NP主要表达iNOS ,分布在黏膜上皮、腺体和血管内皮细胞以及炎症细胞中 ,其产生的大量一氧化氮在NP的发病过程中可能起着重要的作用

Expression of nitric oxide synthase and its significance in nasal polyps

OBJECTIVE: To study the expression of nitric oxide synthase (NOS) and its activity in nasal polyps and its significance. METHOD: Septectomy and immunohistochemisty were used to examine NOS activity and identify the expression of inducible NOS (iNOS) and endotheial NOS (eNOS) in human nasal specimens from patients undergoing nasal spectrumtomy (n = 20) or nasal polypectomy (n = 30). RESULT: The activity of NOS was significantly higher in nasal polyps than in nasal mucosa (4.079 +/- 0.655) U/Pro vs (1.526 +/- 0.310) U/Pro (P<0.01). iNOS reaction product was located in epithelial cells, vascular endothelial cells, nasal glands and inflammation cells of nasal polyps. iNOS reaction product almost was not be founded in nasal mucosa. Amount of. iNOS positive cells in nasal polyps was higher than in nasal mucosa (P<0.01). eNOS reaction product were located in nasal polyps and nasal mucosa. There was no quantitative difference of eNOS between in nasal polyps and nasal mucosa. Amount of iNOS positive cells was significantly higher than of eNOS positive cells in nasal polyps (P<0.01). CONCLUSION: iNOS, mainly expressed in nasal polyps, locates in epithelial cells and vascular endothelial cells and nasal glands and inflammation cells in nasal polyps, which produce NO at high concentration that plays an important role in the formation of nasal polyps.