| 羧甲基葡聚糖体外抗肿瘤作用的研究 |
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| 引用本文： | 孙翠玲,杨文鸽,李花霞,童丽娟,潘云娣. 羧甲基葡聚糖体外抗肿瘤作用的研究[J]. 中国生化药物杂志, 2006, 27(3): 153-155 |
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| 作者姓名： | 孙翠玲 杨文鸽 李花霞 童丽娟 潘云娣 |
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| 作者单位： | 1. 宁波大学,生命科学与生物工程学院,浙江,宁波,315211
2. 中国铝业股份有限公司,郑州研究院,河南,郑州,450041 |
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| 基金项目： | 浙江省宁波市科技局资助项目 |
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| 摘 要： | 目的研究不同取代度羧甲基葡聚糖(CMG)的体外抗肿瘤作用。方法以HL-7702正常肝细胞、HepG-2肝癌细胞、MGC-803胃癌细胞为实验材料,通过四甲基偶氮唑盐(MTT)比色法分析。结果各种取代度的CMG对HL-7702的生长影响较小,但对HepG-2和MGC-803的抑制作用明显,并且随浓度升高对HepG-2、MGC-803的抑制作用也随之增强,尤以取代度为0.556的CMG2最为显著。CMG2浓度达到500μg/mL时对HepG-2的生长抑制率为54.3%,浓度为1 000μg/mL时对MGC-803抑制率达到51.3%。结论在体外实验中,取代度0.55左右的CMG能有效抑制HepG-2肝癌细胞、MGC-803胃癌细胞的生长。
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| 关 键 词： | 羧甲基葡聚糖 取代度 四甲基偶氮唑盐 抗肿瘤 |
| 文章编号： | 1005-1678（2006）03-0153-03 |
| 收稿时间： | 2005-08-02 |
| 修稿时间： | 2005-11-06 |
The anti-cancer effect of carboxymethylglucan in vitro |
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| SUN Cui-ling,YANG Wen-ge,LI Hua-xia,TONG Li-juan,PAN Yun-di. The anti-cancer effect of carboxymethylglucan in vitro[J]. Chinese Journal of Biochemical Pharmaceutics, 2006, 27(3): 153-155 |
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| Authors: | SUN Cui-ling YANG Wen-ge LI Hua-xia TONG Li-juan PAN Yun-di |
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| Affiliation: | 1. School of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China ; 2. Zhengzhou Research Institute of China Aluminum Corporation, Zhengzhou 450041, China |
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| Abstract: | Purpose To detect the anti-cancer effect of carboxymethylg-lucan(CMG) with different degree of substitution(DS)in vitro.MethodsUsing HL-7702,HepG-2 and MGC-803 cell as the experimental objects,the anti-cancer effect of CMG was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) colorimetric Method.ResultsCMG weakly inhibited the growth of HL-7702,but obviously affected the growth of HepG-2 and MGC-803.The higher concentration of CMG,the bigger inhibitory effect on HepG-2 and MGC-803 cell is.The inhibitory ratio of CMG2 to HepG-2 was 54.3% at the concentration of 500 μg/mL,to MGC-803 was 51.3% at the concentration of 1 000 μg/mL.ConclusionCMG(DS≈0.55) could effectively inhibit the growth of HepG-2 and MGC-803 cell. |
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| Keywords: | carboxymethylglucan degree of substitution MTT anti-cancer |
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