| HPLC测定九里香中脱水长叶九里香内酯的含量 |
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| 引用本文： | 姜平川,李嘉,杨海船,张赟赟,李红,黄建猷. HPLC测定九里香中脱水长叶九里香内酯的含量[J]. 中国现代应用药学, 2011, 28(4): 341-344 |
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| 作者姓名： | 姜平川 李嘉 杨海船 张赟赟 李红 黄建猷 |
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| 作者单位： | 广西壮族自治区中医药研究院，南宁 530022;广西壮族自治区中医药研究院，南宁 530022;广西壮族自治区中医药研究院，南宁 530022;广西壮族自治区中医药研究院，南宁 530022;广西壮族自治区中医药研究院，南宁 530022;广西壮族自治区中医药研究院，南宁 530022 |
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| 基金项目： | 广西科技厅科技攻关项目(桂科攻0815005-1-22) |
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| 摘 要： | 目的建立九里香药材中脱水长叶九里香内酯的含量测定方法。方法采用高效液相色谱法,使用Agilent EsclipeXDB-C18色谱柱（150 mm×4.6 mm,5μm）,流动相为甲醇-水（55∶45）,流速1 mL.min-1,检测波长332 nm,柱温25℃。结果脱水长叶九里香内酯进样浓度在20.72～207.2μg.mL-1内,与峰面积呈良好的线性关系（r=0.999 8）;其平均回收率为98.41%,RSD为1.65%（n=6）。结论本法简便易行,重复性好,可用于九里香药材中脱水长叶九里香内酯的含量测定。
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| 关 键 词： | 九里香 脱水长叶九里香内酯 高效液相色谱法 含量测定 |
| 收稿时间： | 2010-06-23 |
Determination of Phebalosin in Murrayae Folium et Cacumen by HPLC |
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| JIANG Pingchuan,LI Ji,YANG Haichuan,ZHANG Yunyun,LI Hong and HUANG Jianyou. Determination of Phebalosin in Murrayae Folium et Cacumen by HPLC[J]. The Chinese Journal of Modern Applied Pharmacy, 2011, 28(4): 341-344 |
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| Authors: | JIANG Pingchuan LI Ji YANG Haichuan ZHANG Yunyun LI Hong HUANG Jianyou |
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| Affiliation: | Guangxi Institute of Chinese Medicine & Pharmaceutical Science, Nanning 530022, China;Guangxi Institute of Chinese Medicine & Pharmaceutical Science, Nanning 530022, China;Guangxi Institute of Chinese Medicine & Pharmaceutical Science, Nanning 530022, China;Guangxi Institute of Chinese Medicine & Pharmaceutical Science, Nanning 530022, China;Guangxi Institute of Chinese Medicine & Pharmaceutical Science, Nanning 530022, China;Guangxi Institute of Chinese Medicine & Pharmaceutical Science, Nanning 530022, China |
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| Abstract: | OBJECTIVE To establish a high performance liquid chromatographic method for the determination of phebalosin in Murrayae Folium et Cacumen.METHODS The analysis was carried out on an Agilent Esclipe XDB-C18 column(150 mm×4.6 mm,5 μm),using a mixture of 55% methanol and 45% water as the mobile phase at a flow rate of 1 mL·min-1 at 25 ℃,the wavelength for measurement was 332 nm.RESULTS The calibration curve was linear in the range of 20.72·207.2 μg·mL·1(r=0.999 8);the average recovery was 98.41% with RSD of 1.65%(n=6).CONCLUSION The method is simple,accurate and reproducible,and it is suitable for the determination of phebalosin in Murrayae Folium et Cacumen. |
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| Keywords: | Murrayae Folium et Cacumen phebalosin HPLC determination |
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