重组大鼠肝再生增强因子对急性肾衰竭大鼠肾脏的保护作用

目的 探讨重组大鼠肝再生增强因子(rrALR)对庆大霉素所致急性肾衰竭（ARF）大鼠肾小管上皮细胞及肾功能的保护作用。 方法 雌性Wistar大鼠150只，随机分成5组，每组30只，即健康对照组，ARF模型组，模型+空质粒对照组（空质粒组），模型+rrALR干预组（ALR组）：根据给予rrALR的剂量不同分为ALR1组和ALR2组两个亚组。分别于实验的第4、8、12、16和21天每组随机抽取6只大鼠在留取血、尿标本后，处死大鼠并取肾组织标本。常规生化方法检测各组大鼠BUN、Scr和尿N-乙酰-β-D-葡萄糖苷酶（NAG）酶的变化；PAS染色观察各组大鼠肾组织病理学改变；免疫组化法检测大鼠肾组织中ALR和增殖细胞核抗原（PCNA）的表达；Western印迹法检测肾组织中ALR蛋白的表达量。 结果 ARF大鼠各组BUN、Scr及尿NAG酶水平在第4、8、12、16天时均较对照组显著升高（P < 0.05）。与模型组和空质粒组相比，ALR组BUN、Scr及尿NAG酶水平明显降低（P < 0.05）；肾组织病理损害程度在各时间点明显减轻；而肾组织的ALR蛋白表达增加（P < 0.05）；肾小管上皮细胞增殖活跃；PCNA阳性细胞呈弥漫性分布，增殖指数（PI）明显升高（P < 0.05）。 结论 rrALR对急性损伤的肾小管上皮细胞具有减轻病变和促进再生修复的作用，可明显改善ARF大鼠的肾功能。

Protective effect of recombination rat augmenter of liver regeneration on kidney in acute renal failure rats

Objective To investigate the protective effects of recombination rat augmenter of liver regeneration (rrALR) on tubular cell injury and renal dysfunction in rats with acute renal failure (ARF)induced by gentamicin. Methods One hundred fifty female Wistar rots were randomly divided into 5 groups: group A (control), group B (gentamincin 140 mg/kg+ saline 100 μg/kg), group C (gentarnincin 140 mg/kg +blank plasmid 100 μg/kg), group Dl (gentaminein 140 mg/kg+rrALR 80 μg/kg), group D2 (gentamincin 140 mg/kg +rrALR 160 μg/kg). Rats were sacrificed at day 4, 8, 12, 16 and 21 after gentamicin first injection. Blood urea nitrogen (BUN), serum creatinine (Scr) and urine N-aeetyl-β-D-glucosaminidase (UNAG) were measured. The histopathologic changes were observed by periodic aeid-Schiff (PAS) staining. Protein expression of ALR and PCNA in renal tissue was detected using immtmohistochemistry and Western blot. Results Compared with control rots, the levels of BUN, Scr and UNAG were significantly increased in ARF rats at day 4, 8, 12 and 16 (P<0.05), however, the renal dysfunction and the renal histopathologieal injury were significantly improved in ARF rats simultaneously administered with rrALR (group D) compared with ARF rats (group B and C). The protein level of ALR, the number of PCNA positive tubular ceils and the proliferation index (PI) in group D were significantly higher than those of group B and C (P<0.05). Conclusion rrALR can promote the regeneration of tubular cells in nephrotoxie ARF rats and ameliorate renal dysfunction.