| 抗纤维化短肽对血清诱导的心脏成纤维细胞增殖和胶原合成与I、III型胶原表达抑制作用的实验研究 |
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| 引用本文： | 马文东,张丽娟,罗玲,裴新,户万秘,杨方. 抗纤维化短肽对血清诱导的心脏成纤维细胞增殖和胶原合成与I、III型胶原表达抑制作用的实验研究[J]. 华北煤炭医学院学报, 2008, 0(3) |
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| 作者姓名： | 马文东 张丽娟 罗玲 裴新 户万秘 杨方 |
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| 作者单位： | 华北煤炭医学院实验中心 华北煤炭医学院实验中心 河北唐山 河北唐山 |
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| 基金项目： | 河北省科技厅博士基金(04547002D-5)资助项目;; 河北省教育厅基金(2003112)资助项目;; 唐山市新医药基础研究重点实验室项目(04362001B) |
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| 摘 要： | ①目的探讨N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸(AcSDKP)对不同浓度血清(FBS)诱导的大鼠心脏成纤维细胞增殖和胶原合成的调节作用。②方法选用新生大鼠心脏成纤维细胞;采用3H-TdR掺入法检测心脏成纤维细胞的增殖;采用3H-脯氨酸掺入法检测心脏成纤维细胞胶原的合成,采用westernblot法检测心脏成纤维细胞I型与III型胶原的表达。③结果在0.4%、2%和10%几种血清浓度的条件下,随着血清浓度的增加,反映心脏成纤维细胞增殖3H-TdR掺入量和胶原合成的3H-脯氨酸掺入量增加。表明一定浓度的血清能诱导心脏成纤维细胞增殖和胶原合成。与0.4%血清的基础培养条件相比较,10%高浓度血清能刺激心脏成纤维细胞I、III型胶原蛋白的表达。在10-10~10-8mol/L浓度范围内,AcSDKP对10%诱导的心脏成纤维细胞增殖和胶原合成有抑制作用,并且在10-9mol/L时,AcSDKP对心脏成纤维细胞增殖、胶原合成抑制作用最强。同时10-9mol/L的AcSDKP对心脏成纤维细胞I、III型胶原蛋白的表达也有明显的抑制作用。④结论AcSDKP对血清介导的心脏成纤维细胞增殖、胶原合成以及I、III型胶原蛋...
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| 关 键 词： | N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸 血清 心脏成纤维细胞 胶原 |
Study for inhibitory effect of anti-fibrosis short peptide on proliferation and the collagen synthesis and expression in cultured rat cardiac fibroblast mediated by FBS |
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| Ma Wendong,Zhang Lijuan,Luo Ling,et al. Study for inhibitory effect of anti-fibrosis short peptide on proliferation and the collagen synthesis and expression in cultured rat cardiac fibroblast mediated by FBS[J]. Journal of North China Coal Medical College, 2008, 0(3) |
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| Authors: | Ma Wendong Zhang Lijuan Luo Ling et al |
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| Affiliation: | Ma Wendong; Zhang Lijuan; Luo Ling; et al(Experimental and Research Center; North China Coal Medical University; Tangshan 063000; China ); |
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| Abstract: | Objectives To investigate the effect of N-acetyl-seryl-aspartyl-lysyl-proline (AcSDKP) on proliferation and the collagen synthesis and expression in cultured rat cardiac fibroblasts mediated by fetal bovine serums (FBS).Methods Neonatal rat cardiac fibroblasts were isolated. The cell proliferation was observed by 3H-TdR incorporation assay. The synthesis of collagen was measured by 3H-proline incorporation assay. The expression of type I and type III collagen was detected by western blotting. Results The co... |
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| Keywords: | N-acetyl-seryl-aspartyl-lysyl-proline. Fetal bovine serums. Cardiac fibroblast. Collagen |
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