| UV法和HPLC法测定甘草总黄酮混悬液中总黄酮和查尔酮含量 |
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| 引用本文： | 邢桂珍,陶卿,崔玉梅,廖远军,邓旭明.UV法和HPLC法测定甘草总黄酮混悬液中总黄酮和查尔酮含量[J].安徽医药,2014,0(12):2251-2255. |
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| 作者姓名： | 邢桂珍 陶卿 崔玉梅 廖远军 邓旭明 |
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| 作者单位： | 1. 吉林大学和平校区动物医学学院,吉林长春,130062
2. 广西北斗星动物保健品有限公司,广西南宁,530007 |
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| 基金项目： | 863计划之“十二五”农村领域国家科技计划课题 |
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| 摘 要： | 目的：建立甘草总黄酮混悬液中总黄酮和甘草查尔酮的含量测定方法，为该制剂的质量控制提供依据。方法采用紫外分光光度法（ UV）和高效液相色谱（ HPLC）法测定总黄酮和甘草查尔酮的含量。结果方法准确可靠，专属性强，阴性无干扰。结论通过线性关系、精密度试验、重复性试验及回收率试验等证明所建立的含量测定方法是可行的。
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| 关 键 词： | 甘草总黄酮混悬液 总黄酮 甘草查尔酮 含量测定 |
Determination of general flavone and blicochalcone in glycyrrhiza flavonoids suspension by UV and HPLC |
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| Institution: | XING Gui-zhen;TAO Qing;CUI Yu-mei;Laboratory Animal Center,Jilin University;Guangxi Beidouxing Animal Health Product Co. ,Ltd; |
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| Abstract: | Objective To establish content determination of total flavonoids and licochalcone for glycyrrhiza flavonoids suspension . Methods UV spectrophotometry and HPLC method was used to measure the content of general flavone and licochalcone .Results The method is accurate,reliable,and well specific without interference .Conclusions The method has been proved feasible through lin-ear relation,precision test,reproducibility test,and recovery test. |
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| Keywords: | glycyrrhiza flavonoids suspension flavonoids licochalcone content determination |
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