HPLC法同时测定糖浆剂中三种B族维生素及山梨酸的含量

目的建立高效液相色谱法同时测定多维生素糖浆中盐酸硫胺(VitB1)、核黄素-5′-磷酸钠(VitB2)、盐酸吡多辛(VitB6)及防腐剂山梨酸的含量。方法采用高效液相色谱法,使用Allthna C18柱(4.6×250mm,5μm),流动相A为8mmol·L-1己烷磺酸钠溶液(1000mL溶液加入三乙胺0.25mL和乙酸9.2mL);流动相B为甲醇;梯度洗脱;流速为1.0mL·min-1;检测波长为280nm。结果 VitB1、VitB2、VitB6、山梨酸的浓度分别在0.02～0.4mg·L-1、0.02～0.4mg·mL-1、0.007～0.1mg·.mL-1、0.03～0.6mg·mL-1范围内与峰面积线性关系良好;平均回收率分别为99.2%、101.9%、101.5%、101.5%。结论本实验所建立的高效液相色谱分析方法快速、灵敏,色谱峰分离度良好,结果准确可靠,可作为多维生素糖浆的质量控制项目之一。

Determination of VitB1,VitB2,VitB6 and sorbic acid in Alvityl Syrup by HPLC

OBJECTIVE To develop an HPLC for the determination of VitB1, VitB2, VitB6 and sorbic acid in Alvityl Syrup. METHODS After being diluted with pure water, samples were separated by HPLC on a C18 （4.6× 250mm, 5μm） column with mobile phases of 8mmol ·L^-1 sodium hexanesulfonate solution （1000mL solution containing 0. 25mL triethylamine and 9.2mL acetic acid ）and methanol. The mobile phases were gradient eluted. The detection wavelength was 280nm. RESULTS Calibration curves for VitB1, VitB2, VitB6 and sorbic acid were linear over range of 0.02 -0.4mg · mL^-1 , 0. 02 - 0. 4mg ·mL^-10. 007 - 0. 1mg ·mL^-1 and 0. 03 - 0. 6mg·mL^-1 , respectively. And the average recoveries were 99.2 - 101.9 %, 101.5%,101.5%,respectively. CONCLUSION The assay was proved to be sensitive,accurate and convenient with good resolution. It can be applied to control the quality of Alvityl Syrup.