| 实时荧光RT-PCR定量检测BRMS1基因表达 |
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| 引用本文： | 张英兰,魏让,石红梅,王丽霞,刘秀英,阎建文.实时荧光RT-PCR定量检测BRMS1基因表达[J].中国医疗前沿,2008,3(24):5-6. |
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| 作者姓名： | 张英兰 魏让 石红梅 王丽霞 刘秀英 阎建文 |
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| 作者单位： | 山西省肿瘤医院,山西,太原,030013 |
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| 摘 要： | 目的建立一种实时荧光RT-PCR定量检测乳腺组织中BRMS1mRNA表达水平的方法。方法采明逆转录聚合酶链反应(RT-PCR)扩增目的基因片段,并实时检测产物的荧光强度,根据标准品建立的标准曲线,计算出待测样本中BRMS1mRNA含量,并以BRMS1mRNA与GAPDHmRNA的比值作为BRMS1mRNA的表达水平。结果实时荧光RT-PCR检测BRMS1及GAPDH的线性范围为4×102-4×108拷贝/μl。批内和批间变异系数分别为3.9%-4.6%和4.8%-5.5%。BRMS1mRNA表达范围为0-1.65.结论实时荧光定量检测BRMS1mRNA含量的方法具有较高的灵敏度和较好的重复性。
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| 关 键 词： | BRMS1 基因表达 实时荧光PCR |
Quantitative determination of the expression level of Breast cancer metastasis suppressor 1(BRMS1) by real-time fluorescence quantitative RT-PCR method |
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| Institution: | Zhang Yinglan; Wei Rang; Shi Hongmei; et a(Department of Clinical Laboratory; Shanxi Province Tumor Hospital; Taiyuan 030013; China); |
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| Abstract: | Objective To establish a real time fluorescence RT-PCR for quantitative determination of the expression level of BRMS1mRNA in tissue of breast cancer.Methods The fluorescence of the PCR product was detected continuously during amplification.According to the standard curve created by standard,the expression level of BRMS1 were determined and the results were presented as the ratios of BRMS1mRNA to GAPDHmRNA.Results The detection range of the assay was from 4 ×102-4 ×108copies/μl.The coefficient of variation ... |
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| Keywords: | BRMS1 |
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