细粒棘球绦虫重组BCG-Eg95疫苗诱导小鼠脾细胞亚群变化的研究

目的 探讨细粒棘球绦虫重组BCG-Eg95疫苗免疫和Eg原头节攻击后小鼠脾CD4 和CD8 T淋巴细胞亚群的变化.方法 将细粒棘球绦虫重组BCG-Eg95疫苗采用皮下注射、鼻腔内接种、口服灌胃和肌肉注射4种途径分别免疫Balb/c鼠,免疫后8周用Eg原头节进行攻击感染,感染后18周杀鼠取脾,分离脾细胞,流式细胞仪检测脾CD4 和CD8 T淋巴细胞亚群的百分比,同时设有BCG和PBS对照.结果 疫苗接种组的脾CD4 和CD8 T细胞亚群显著增加,口服接种组和肌肉注射组的CD4 T细胞亚群和CD4 /CD8 亚群比值显著高于皮下注射组和鼻腔内接种组.结论 CD4 T细胞亚群可能与细粒棘球绦虫重组BCG-Eg95疫苗诱导的小鼠抗Eg原头节攻击感染的保护力有关,疫苗口服接种和肌肉注射是两种较好的免疫途径.

Changes of of splenocyte subsets in mice immunized with recombinant BCG-Eg95 vaccine against Echinococcus granulosus

Objective To investigate the changes of splenocyte subsets in mice immunized with recombinant BCG-Eg95 vaccine against Echinococcus granulosus(Eg) after the challenge of Eg protoscoleces. Methods Balb/c mice were subcutaneously, intranasally, orally, and intramuscularly vaccinated with the vaccine respectively. The vaccinated mice were challenged with Eg protoscoleces on 8 W post-vaccination and sacrificed on 18W post-infection to get spleen. The splenocytes were subsequently separated to measure the subsets of CD4 and CD8 T cells by FACsort, with BCG and PBS served as controls. Results In the groups of immunization, CD4 and CD8 subsets increased obviously. CD4 and the ratio of CD4 /CD8 in the oral and intramuscular groups were higher than that in the subcutaneous or intranasal group. Conclusion CD4 T cell may be associated with the protection induced by rBCG-Eg95 vaccine against challenge of Eg protoscoleces. Oral and intramuscular vaccination with this vaccine may be good routes.