The effects of thapsigargin on [Ca2+]i in isolated rat mesenteric artery vascular smooth muscle cells |
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| Authors: | I. Baró D. A. Eisner |
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| Affiliation: | (1) Department of Veterinary Preclinical Sciences, University of Liverpool, P.O. Box 147, L69 3BX Liverpool, UK;(2) Present address: Laboratoire de Physiologie cellulaire, Université Paris XI, bât 443, F-91405 Orsay Cedex, France |
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| Abstract: | Summary The effects of thapsigargin were studied on single cells isolated from side branches of the rat mesenteric artery. Thapsigargin (150 nM) produced a transient increase of [Ca2+]i. This transient rise of [Ca2+]i was unaffected by removing external Ca2+ ions. This suggests that thapsigargin is releasing Ca2+ ions from an intracellular store. In the absence of thapsigargin both noradrenaline and caffeine also produced a transient increase of [Ca2+]i. These increases were abolished by prior exposure to thapsigargin. Correspondingly, the effects of thapsigargin were abolished by prior exposure to caffeine. These results show that thapsigargin releases Ca2+ from the noradrenaline and caffeine-sensitive stores. |
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| Keywords: | Thapsigargin intracellular calcium smooth muscle cell noradrenaline caffeine |
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